Arginylation of β-actin regulates actin cytoskeleton and cell motility

Marina Karakozova, Marina Kozak, Catherine C L Wong, Aaron O. Bailey, John R. Yates, Alexander Mogilner, Henry Zebroski, Anna Kashina

Research output: Contribution to journalArticle

Abstract

Posttranslational arginylation is critical for mouse embryogenesis, cardiovascular development, and angiogenesis, but its molecular effects and the identity of proteins arginylated in vivo are unknown. We found that β-actin was arginylated in vivo to regulate actin filament properties, β-actin localization, and lamella formation in motile cells. Arginylation of β-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo. Thus, posttranslational arginylation of a single protein target can regulate its intracellular function, inducing global changes on the cellular level, and may contribute to cardiovascular development and angiogenesis.

Original languageEnglish (US)
Pages (from-to)192-196
Number of pages5
JournalScience
Volume313
Issue number5784
DOIs
StatePublished - Jul 14 2006

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Actin Cytoskeleton
Cell Movement
Actins
Embryonic Development
Proteins

ASJC Scopus subject areas

  • General

Cite this

Karakozova, M., Kozak, M., Wong, C. C. L., Bailey, A. O., Yates, J. R., Mogilner, A., ... Kashina, A. (2006). Arginylation of β-actin regulates actin cytoskeleton and cell motility. Science, 313(5784), 192-196. https://doi.org/10.1126/science.1129344

Arginylation of β-actin regulates actin cytoskeleton and cell motility. / Karakozova, Marina; Kozak, Marina; Wong, Catherine C L; Bailey, Aaron O.; Yates, John R.; Mogilner, Alexander; Zebroski, Henry; Kashina, Anna.

In: Science, Vol. 313, No. 5784, 14.07.2006, p. 192-196.

Research output: Contribution to journalArticle

Karakozova, M, Kozak, M, Wong, CCL, Bailey, AO, Yates, JR, Mogilner, A, Zebroski, H & Kashina, A 2006, 'Arginylation of β-actin regulates actin cytoskeleton and cell motility', Science, vol. 313, no. 5784, pp. 192-196. https://doi.org/10.1126/science.1129344
Karakozova M, Kozak M, Wong CCL, Bailey AO, Yates JR, Mogilner A et al. Arginylation of β-actin regulates actin cytoskeleton and cell motility. Science. 2006 Jul 14;313(5784):192-196. https://doi.org/10.1126/science.1129344
Karakozova, Marina ; Kozak, Marina ; Wong, Catherine C L ; Bailey, Aaron O. ; Yates, John R. ; Mogilner, Alexander ; Zebroski, Henry ; Kashina, Anna. / Arginylation of β-actin regulates actin cytoskeleton and cell motility. In: Science. 2006 ; Vol. 313, No. 5784. pp. 192-196.
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