Arbitrarily primed DNA fingerprinting for phylogenetic reconstruction in vertebrates

The Xiphophorus model

R. L. Borowsky, M. McClelland, R. Cheng, J. Welsh

Research output: Contribution to journalArticle

Abstract

Arbitrarily primed PCR (AP-PCR or RAPD) is a technique for producing species-specific DNA fingerprints. We tested the utility of AP-PCR as a source of phylogenetically informative characters in three separate experiments, using fishes of the genus Xiphophorus. We chose Xiphophorus as a standard of comparison, because evolutionary relationships within the genus have been studied repeatedly using a variety of techniques. We compared our results to a 'classical' phylogenetic hypothesis synthesized from studies using morphological, pigmentation, and allozyme characters, and to a recent conflicting hypothesis constructed from DNA sequence data. The sequence- based hypothesis places the southern swordtail Xiphophorus clemenciae squarely within the platyfish, whereas the classical hypothesis separates the two groups. In addition, the two hypotheses differ in their clustering of species of northern swordtails. Our findings are in close accord with the classical hypothesis. Our results allow the strongest phylogenetic hypothesis yet for Xiphophorus and demonstrate the utility of AP-PCR for studying species relationships within vertebrate genera.

Original languageEnglish (US)
Pages (from-to)1022-1032
Number of pages11
JournalMolecular Biology and Evolution
Volume12
Issue number6
StatePublished - 1995

Fingerprint

Cyprinodontiformes
Xiphophorus
DNA fingerprinting
DNA Fingerprinting
DNA sequences
Fish
Isoenzymes
Vertebrates
vertebrate
vertebrates
phylogenetics
DNA
phylogeny
Polymerase Chain Reaction
Experiments
allozyme
Pigmentation
Cluster Analysis
fish
Fishes

Keywords

  • AP-PCR
  • phylogeny
  • RAPD
  • Xiphophorus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

Arbitrarily primed DNA fingerprinting for phylogenetic reconstruction in vertebrates : The Xiphophorus model. / Borowsky, R. L.; McClelland, M.; Cheng, R.; Welsh, J.

In: Molecular Biology and Evolution, Vol. 12, No. 6, 1995, p. 1022-1032.

Research output: Contribution to journalArticle

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