Aragonite-associated biomineralization proteins are disordered and contain interactive motifs

Research output: Contribution to journalArticle

Abstract

Motivation: The formation of aragonite mineral in the mollusk shell or pearl nacre requires the participation of a diverse set of proteins that form the mineralized extracellular matrix. Although self-assembly processes have been identified for several nacre proteins, these proteins do not contain known globular protein-protein binding domains. Thus, we hypothesize that other sequence features are responsible for nacre matrix protein-protein assembly processes and ultimately aragonite biosynthesis.Results: Of 39 mollusk aragonite-associated protein sequences, 100% contain at least one region of intrinsic disorder or unfolding, with the highest percentages found in framework and pearl-associated proteins relative to the intracrystalline proteins. In some instances, these intrinsically disordered regions were identified as bind/fold sequences, and a limited number correlate with known biomineral-relevant sequences. Interestingly, 95% of the aragonite-associated protein sequences were found to contain at least one occurrence of amyloid-like or cross-β strand aggregation-prone supersecondary motifs, and this correlates with known aggregation and aragonite formation functions in three experimentally tested protein sequences. Collectively, our findings indicate that aragonite-associated proteins have evolved signature sequence traits of intrinsic disorder and aggregation-prone regions that are important for their role(s) in matrix assembly and mineralization.

Original languageEnglish (US)
Pages (from-to)3182-3185
Number of pages4
JournalBioinformatics
Volume28
Issue number24
DOIs
StatePublished - Dec 2012

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Biomineralization
Calcium Carbonate
Proteins
Protein
Nacre
Protein Sequence
Aggregation
Agglomeration
Mollusca
Correlate
Disorder
Biosynthesis
Self-assembly
Unfolding
Percentage
Shell
Amyloid
Protein Binding
Fold
Signature

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Computational Theory and Mathematics
  • Computer Science Applications
  • Computational Mathematics
  • Statistics and Probability
  • Medicine(all)

Cite this

Aragonite-associated biomineralization proteins are disordered and contain interactive motifs. / Evans, John.

In: Bioinformatics, Vol. 28, No. 24, 12.2012, p. 3182-3185.

Research output: Contribution to journalArticle

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