Arabidopsis TCH3 encodes a novel Ca2+ binding protein and shows environmentally induced and tissue-specific regulation

M. L. Sistrunk, D. M. Antosiewicz, M. M. Purugganan, J. Braam

Research output: Contribution to journalArticle

Abstract

The Arabidopsis touch (TCH) genes are up-regulated in response to various environmental stimuli, including touch, wind, and darkness. Previously, it was determined that TCH1 encodes a calmodulin; TCH2 and TCH3 encode calmodulin-related proteins. Here, we present the sequence and genomic organization of TCH3. TCH3 is composed of three repeats; remarkably, the first two repeats share 94% sequence identity, including introns that are 99% identical. The conceptual TCH3 product is 58 to 60% identical to known Arabidopsis calmodulins; however, unlike calmodulin, which has four Ca2+ binding sites, TCH3 has six potential Ca2+ binding domains. TCH3 is capable of binding Ca2+, as demonstrated by a Ca(2+)-specific shift in electrophoretic mobility. 5' Fragments of the TCH3 locus, when fused to the beta-glucuronidase (GUS) reporter gene, are sufficient to confer inducibility of expression following stimulation of plants with touch or darkness. These TCH3 sequences also direct expression to growing regions of roots, vascular tissue, root/shoot junctions, trichomes, branch points of the shoot, and regions of siliques and flowers. The pattern of expression of the TCH3/GUS reporter genes most likely reflects expression of the native TCH3 gene, because immunostaining of the TCH3 protein shows similar localization. The tissue-specific expression of TCH3 suggests that expression may be regulated not only by externally applied mechanical stimuli but also by mechanical stresses generated during development. Consequently, TCH3 may perform a Ca(2+)-modulated function involved in generating changes in cells and/or tissues that result in greater strength or flexibility.
Original languageUndefined
Pages (from-to)1553-65
JournalPlant Cell
Volume6
Issue number11
DOIs
StatePublished - 1994

Keywords

  • Amino Acid Sequence Arabidopsis/*genetics/growth & development *Arabidopsis Proteins Base Sequence Calcium/*metabolism Calcium-Binding Proteins/biosynthesis/*genetics Calmodulin/genetics Darkness *Gene Expression Regulation, Plant Genes, Plant/*genetics Genes, Reporter Molecular Sequence Data Physical Stimulation Plant Proteins/biosynthesis/*genetics Plants, Genetically Modified RNA, Messenger/analysis Recombinant Fusion Proteins/biosynthesis Sequence Analysis, DNA Sequence Homology, Amino Acid Signal Transduction Tissue Distribution

Cite this

Arabidopsis TCH3 encodes a novel Ca2+ binding protein and shows environmentally induced and tissue-specific regulation. / Sistrunk, M. L.; Antosiewicz, D. M.; Purugganan, M. M.; Braam, J.

In: Plant Cell, Vol. 6, No. 11, 1994, p. 1553-65.

Research output: Contribution to journalArticle

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title = "Arabidopsis TCH3 encodes a novel Ca2+ binding protein and shows environmentally induced and tissue-specific regulation",
abstract = "The Arabidopsis touch (TCH) genes are up-regulated in response to various environmental stimuli, including touch, wind, and darkness. Previously, it was determined that TCH1 encodes a calmodulin; TCH2 and TCH3 encode calmodulin-related proteins. Here, we present the sequence and genomic organization of TCH3. TCH3 is composed of three repeats; remarkably, the first two repeats share 94{\%} sequence identity, including introns that are 99{\%} identical. The conceptual TCH3 product is 58 to 60{\%} identical to known Arabidopsis calmodulins; however, unlike calmodulin, which has four Ca2+ binding sites, TCH3 has six potential Ca2+ binding domains. TCH3 is capable of binding Ca2+, as demonstrated by a Ca(2+)-specific shift in electrophoretic mobility. 5' Fragments of the TCH3 locus, when fused to the beta-glucuronidase (GUS) reporter gene, are sufficient to confer inducibility of expression following stimulation of plants with touch or darkness. These TCH3 sequences also direct expression to growing regions of roots, vascular tissue, root/shoot junctions, trichomes, branch points of the shoot, and regions of siliques and flowers. The pattern of expression of the TCH3/GUS reporter genes most likely reflects expression of the native TCH3 gene, because immunostaining of the TCH3 protein shows similar localization. The tissue-specific expression of TCH3 suggests that expression may be regulated not only by externally applied mechanical stimuli but also by mechanical stresses generated during development. Consequently, TCH3 may perform a Ca(2+)-modulated function involved in generating changes in cells and/or tissues that result in greater strength or flexibility.",
keywords = "Amino Acid Sequence Arabidopsis/*genetics/growth & development *Arabidopsis Proteins Base Sequence Calcium/*metabolism Calcium-Binding Proteins/biosynthesis/*genetics Calmodulin/genetics Darkness *Gene Expression Regulation, Plant Genes, Plant/*genetics Genes, Reporter Molecular Sequence Data Physical Stimulation Plant Proteins/biosynthesis/*genetics Plants, Genetically Modified RNA, Messenger/analysis Recombinant Fusion Proteins/biosynthesis Sequence Analysis, DNA Sequence Homology, Amino Acid Signal Transduction Tissue Distribution",
author = "Sistrunk, {M. L.} and Antosiewicz, {D. M.} and Purugganan, {M. M.} and J. Braam",
note = "Sistrunk, M L Antosiewicz, D M Purugganan, M M Braam, J GMO7833/GM/NIGMS NIH HHS/United States R29 GM46346/GM/NIGMS NIH HHS/United States Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. United states Plant Cell. 1994 Nov;6(11):1553-65.",
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T1 - Arabidopsis TCH3 encodes a novel Ca2+ binding protein and shows environmentally induced and tissue-specific regulation

AU - Sistrunk, M. L.

AU - Antosiewicz, D. M.

AU - Purugganan, M. M.

AU - Braam, J.

N1 - Sistrunk, M L Antosiewicz, D M Purugganan, M M Braam, J GMO7833/GM/NIGMS NIH HHS/United States R29 GM46346/GM/NIGMS NIH HHS/United States Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. United states Plant Cell. 1994 Nov;6(11):1553-65.

PY - 1994

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N2 - The Arabidopsis touch (TCH) genes are up-regulated in response to various environmental stimuli, including touch, wind, and darkness. Previously, it was determined that TCH1 encodes a calmodulin; TCH2 and TCH3 encode calmodulin-related proteins. Here, we present the sequence and genomic organization of TCH3. TCH3 is composed of three repeats; remarkably, the first two repeats share 94% sequence identity, including introns that are 99% identical. The conceptual TCH3 product is 58 to 60% identical to known Arabidopsis calmodulins; however, unlike calmodulin, which has four Ca2+ binding sites, TCH3 has six potential Ca2+ binding domains. TCH3 is capable of binding Ca2+, as demonstrated by a Ca(2+)-specific shift in electrophoretic mobility. 5' Fragments of the TCH3 locus, when fused to the beta-glucuronidase (GUS) reporter gene, are sufficient to confer inducibility of expression following stimulation of plants with touch or darkness. These TCH3 sequences also direct expression to growing regions of roots, vascular tissue, root/shoot junctions, trichomes, branch points of the shoot, and regions of siliques and flowers. The pattern of expression of the TCH3/GUS reporter genes most likely reflects expression of the native TCH3 gene, because immunostaining of the TCH3 protein shows similar localization. The tissue-specific expression of TCH3 suggests that expression may be regulated not only by externally applied mechanical stimuli but also by mechanical stresses generated during development. Consequently, TCH3 may perform a Ca(2+)-modulated function involved in generating changes in cells and/or tissues that result in greater strength or flexibility.

AB - The Arabidopsis touch (TCH) genes are up-regulated in response to various environmental stimuli, including touch, wind, and darkness. Previously, it was determined that TCH1 encodes a calmodulin; TCH2 and TCH3 encode calmodulin-related proteins. Here, we present the sequence and genomic organization of TCH3. TCH3 is composed of three repeats; remarkably, the first two repeats share 94% sequence identity, including introns that are 99% identical. The conceptual TCH3 product is 58 to 60% identical to known Arabidopsis calmodulins; however, unlike calmodulin, which has four Ca2+ binding sites, TCH3 has six potential Ca2+ binding domains. TCH3 is capable of binding Ca2+, as demonstrated by a Ca(2+)-specific shift in electrophoretic mobility. 5' Fragments of the TCH3 locus, when fused to the beta-glucuronidase (GUS) reporter gene, are sufficient to confer inducibility of expression following stimulation of plants with touch or darkness. These TCH3 sequences also direct expression to growing regions of roots, vascular tissue, root/shoot junctions, trichomes, branch points of the shoot, and regions of siliques and flowers. The pattern of expression of the TCH3/GUS reporter genes most likely reflects expression of the native TCH3 gene, because immunostaining of the TCH3 protein shows similar localization. The tissue-specific expression of TCH3 suggests that expression may be regulated not only by externally applied mechanical stimuli but also by mechanical stresses generated during development. Consequently, TCH3 may perform a Ca(2+)-modulated function involved in generating changes in cells and/or tissues that result in greater strength or flexibility.

KW - Amino Acid Sequence Arabidopsis/genetics/growth & development Arabidopsis Proteins Base Sequence Calcium/metabolism Calcium-Binding Proteins/biosynthesis/genetics Calmodulin/genetics Darkness Gene Expression Regulation, Plant Genes, Plant/genetics Genes,

U2 - 10.1105/tpc.6.11.1553

DO - 10.1105/tpc.6.11.1553

M3 - Article

VL - 6

SP - 1553

EP - 1565

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 11

ER -