Aquatic effect duration study of Cry4 toxin with immunoassay and Aedes aegypti larval biotest

Ágnes Fejes, Eszter Takács, Gábor Fekete, Béla Darvas, Bruce S. Ferguson, Deepak Saxena, András Székács

Research output: Contribution to journalArticle

Abstract

Bacillus thuringiensis subsp. israelensis (Bti) preparations are widely used for culicid larvae. There is no suitable commercially available analytical method for Cry4 toxin as active ingredient in Bti preparations. To overcome this limitation, an enzyme-linked immunosorbent assay (ELISA) was developed for quantitative determination of Cry4 toxin allowing a limit of detection (LOD) of ~2 ng ml-1 in water. Preconcentration of aqueous samples by lyophilisation resulted in low but reproducible recoveries (25.7±6.8%), and the practical LODs for Bti preparations VECTOBAC WDG granulate and VECTOBAC 12 AS suspension were found to be ~170 ng ml-1 and ~900 ng ml-1, respectively. ELISA determinations indicated a rapid decay in detectable concentrations of VECTOBAC WDG applied at 400 ng ml-1 concentration in surface water: detected concentrations decreased by 18% and 44% in 4 days in water collected from two locations, and dropped below LOD afterwards. Larval mortality of Aedes aegypti indicated a continuous decrease even thereafter. Thus, quantitative Cry4 toxin detection facilitates proper timing and frequency of treatments to achieve optimal efficacy.

Original languageEnglish (US)
Pages (from-to)207-222
Number of pages16
JournalAquatic Insects
Volume34
Issue numberSUPPL. 1
DOIs
StatePublished - Jun 2012

Fingerprint

Bacillus thuringiensis subsp. israelensis
immunoassay
Aedes aegypti
immunoassays
toxin
toxins
duration
detection limit
enzyme-linked immunosorbent assay
assay
enzyme
water
freeze drying
active ingredients
analytical methods
analytical method
surface water
deterioration
larva
mortality

Keywords

  • Aedes aegypti
  • Bacillus thuringiensis subsp. israelensis
  • biotest
  • Cry4 toxin
  • ELISA
  • mosquito larva

ASJC Scopus subject areas

  • Insect Science
  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

Fejes, Á., Takács, E., Fekete, G., Darvas, B., Ferguson, B. S., Saxena, D., & Székács, A. (2012). Aquatic effect duration study of Cry4 toxin with immunoassay and Aedes aegypti larval biotest. Aquatic Insects, 34(SUPPL. 1), 207-222. https://doi.org/10.1080/01650424.2012.643062

Aquatic effect duration study of Cry4 toxin with immunoassay and Aedes aegypti larval biotest. / Fejes, Ágnes; Takács, Eszter; Fekete, Gábor; Darvas, Béla; Ferguson, Bruce S.; Saxena, Deepak; Székács, András.

In: Aquatic Insects, Vol. 34, No. SUPPL. 1, 06.2012, p. 207-222.

Research output: Contribution to journalArticle

Fejes, Á, Takács, E, Fekete, G, Darvas, B, Ferguson, BS, Saxena, D & Székács, A 2012, 'Aquatic effect duration study of Cry4 toxin with immunoassay and Aedes aegypti larval biotest', Aquatic Insects, vol. 34, no. SUPPL. 1, pp. 207-222. https://doi.org/10.1080/01650424.2012.643062
Fejes, Ágnes ; Takács, Eszter ; Fekete, Gábor ; Darvas, Béla ; Ferguson, Bruce S. ; Saxena, Deepak ; Székács, András. / Aquatic effect duration study of Cry4 toxin with immunoassay and Aedes aegypti larval biotest. In: Aquatic Insects. 2012 ; Vol. 34, No. SUPPL. 1. pp. 207-222.
@article{37c624711c674999b3c4372c767d0807,
title = "Aquatic effect duration study of Cry4 toxin with immunoassay and Aedes aegypti larval biotest",
abstract = "Bacillus thuringiensis subsp. israelensis (Bti) preparations are widely used for culicid larvae. There is no suitable commercially available analytical method for Cry4 toxin as active ingredient in Bti preparations. To overcome this limitation, an enzyme-linked immunosorbent assay (ELISA) was developed for quantitative determination of Cry4 toxin allowing a limit of detection (LOD) of ~2 ng ml-1 in water. Preconcentration of aqueous samples by lyophilisation resulted in low but reproducible recoveries (25.7±6.8{\%}), and the practical LODs for Bti preparations VECTOBAC WDG granulate and VECTOBAC 12 AS suspension were found to be ~170 ng ml-1 and ~900 ng ml-1, respectively. ELISA determinations indicated a rapid decay in detectable concentrations of VECTOBAC WDG applied at 400 ng ml-1 concentration in surface water: detected concentrations decreased by 18{\%} and 44{\%} in 4 days in water collected from two locations, and dropped below LOD afterwards. Larval mortality of Aedes aegypti indicated a continuous decrease even thereafter. Thus, quantitative Cry4 toxin detection facilitates proper timing and frequency of treatments to achieve optimal efficacy.",
keywords = "Aedes aegypti, Bacillus thuringiensis subsp. israelensis, biotest, Cry4 toxin, ELISA, mosquito larva",
author = "{\'A}gnes Fejes and Eszter Tak{\'a}cs and G{\'a}bor Fekete and B{\'e}la Darvas and Ferguson, {Bruce S.} and Deepak Saxena and Andr{\'a}s Sz{\'e}k{\'a}cs",
year = "2012",
month = "6",
doi = "10.1080/01650424.2012.643062",
language = "English (US)",
volume = "34",
pages = "207--222",
journal = "Aquatic Insects",
issn = "0165-0424",
publisher = "Taylor and Francis Ltd.",
number = "SUPPL. 1",

}

TY - JOUR

T1 - Aquatic effect duration study of Cry4 toxin with immunoassay and Aedes aegypti larval biotest

AU - Fejes, Ágnes

AU - Takács, Eszter

AU - Fekete, Gábor

AU - Darvas, Béla

AU - Ferguson, Bruce S.

AU - Saxena, Deepak

AU - Székács, András

PY - 2012/6

Y1 - 2012/6

N2 - Bacillus thuringiensis subsp. israelensis (Bti) preparations are widely used for culicid larvae. There is no suitable commercially available analytical method for Cry4 toxin as active ingredient in Bti preparations. To overcome this limitation, an enzyme-linked immunosorbent assay (ELISA) was developed for quantitative determination of Cry4 toxin allowing a limit of detection (LOD) of ~2 ng ml-1 in water. Preconcentration of aqueous samples by lyophilisation resulted in low but reproducible recoveries (25.7±6.8%), and the practical LODs for Bti preparations VECTOBAC WDG granulate and VECTOBAC 12 AS suspension were found to be ~170 ng ml-1 and ~900 ng ml-1, respectively. ELISA determinations indicated a rapid decay in detectable concentrations of VECTOBAC WDG applied at 400 ng ml-1 concentration in surface water: detected concentrations decreased by 18% and 44% in 4 days in water collected from two locations, and dropped below LOD afterwards. Larval mortality of Aedes aegypti indicated a continuous decrease even thereafter. Thus, quantitative Cry4 toxin detection facilitates proper timing and frequency of treatments to achieve optimal efficacy.

AB - Bacillus thuringiensis subsp. israelensis (Bti) preparations are widely used for culicid larvae. There is no suitable commercially available analytical method for Cry4 toxin as active ingredient in Bti preparations. To overcome this limitation, an enzyme-linked immunosorbent assay (ELISA) was developed for quantitative determination of Cry4 toxin allowing a limit of detection (LOD) of ~2 ng ml-1 in water. Preconcentration of aqueous samples by lyophilisation resulted in low but reproducible recoveries (25.7±6.8%), and the practical LODs for Bti preparations VECTOBAC WDG granulate and VECTOBAC 12 AS suspension were found to be ~170 ng ml-1 and ~900 ng ml-1, respectively. ELISA determinations indicated a rapid decay in detectable concentrations of VECTOBAC WDG applied at 400 ng ml-1 concentration in surface water: detected concentrations decreased by 18% and 44% in 4 days in water collected from two locations, and dropped below LOD afterwards. Larval mortality of Aedes aegypti indicated a continuous decrease even thereafter. Thus, quantitative Cry4 toxin detection facilitates proper timing and frequency of treatments to achieve optimal efficacy.

KW - Aedes aegypti

KW - Bacillus thuringiensis subsp. israelensis

KW - biotest

KW - Cry4 toxin

KW - ELISA

KW - mosquito larva

UR - http://www.scopus.com/inward/record.url?scp=84866462204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866462204&partnerID=8YFLogxK

U2 - 10.1080/01650424.2012.643062

DO - 10.1080/01650424.2012.643062

M3 - Article

VL - 34

SP - 207

EP - 222

JO - Aquatic Insects

JF - Aquatic Insects

SN - 0165-0424

IS - SUPPL. 1

ER -