Aptamer-based sensor arrays for the detection and quantitation of proteins

Romy Kirby, Eun Jeong Cho, Brian Gehrke, Travis Bayer, Yoon Sok Park, Dean P. Neikirk, John T. McDevitt, Andrew D. Ellington

Research output: Contribution to journalArticle

Abstract

Aptamer biosensors have been immobilized on beads, introduced into micromachined chips on the electronic tongue sensor array, and used for the detection and quantitation of proteins. Aptamer chips could detect proteins in both capture and sandwich assay formats. Unlike most protein-based arrays, the aptamer chips could be stripped and reused multiple times. The aptamer chips proved to be useful for screening aptamers from in vitro selection experiments and for sensitively quantitating the biothreat agent ricin.

Original languageEnglish (US)
Pages (from-to)4066-4075
Number of pages10
JournalAnalytical chemistry
Volume76
Issue number14
DOIs
StatePublished - Jul 15 2004

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ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Kirby, R., Cho, E. J., Gehrke, B., Bayer, T., Park, Y. S., Neikirk, D. P., McDevitt, J. T., & Ellington, A. D. (2004). Aptamer-based sensor arrays for the detection and quantitation of proteins. Analytical chemistry, 76(14), 4066-4075. https://doi.org/10.1021/ac049858n