Apoptosis of growth plate chondrocytes occurs through a mitochondrial pathway

Cristina Teixeira, A. P P Costas, Yelena Nemelivsky

Research output: Contribution to journalArticle

Abstract

Objective: To determine the role of mitochondria in chondrocyte apoptosis induced by inorganic phosphate (Pi). Materials and Methods: Chondrocytes isolated from the growth plates of chick embryo tibia were treated with Pi in serum-free media; chondrocyte viability, mitochondrial membrane potential, cytochrome c release from mitochondria, caspase 3 activity, endonuclease activity, and DNA fragmentation were investigated. Results: Exposure to Pi for 24 hours induced apoptosis in growth plate chondrocytes through a pathway that involved loss of mitochondrial function, release of cytochrome c into the cytoplasm, increases in caspase 3 and endonuclease activities, and fragmentation of DNA. Conclusions: This study suggests that mitochondria are important players in Pi-induced apoptosis.

Original languageEnglish (US)
Pages (from-to)129-134
Number of pages6
JournalAngle Orthodontist
Volume77
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

Growth Plate
Chondrocytes
Apoptosis
Mitochondria
Endonucleases
DNA Fragmentation
Cytochromes c
Caspase 3
Mitochondrial Membrane Potential
Serum-Free Culture Media
Chick Embryo
Tibia
Cytoplasm
Phosphates

Keywords

  • Apoptosis
  • Caspases
  • Chondrocytes
  • Endonucleases
  • Growth plate
  • Inorganic phosphate

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Apoptosis of growth plate chondrocytes occurs through a mitochondrial pathway. / Teixeira, Cristina; Costas, A. P P; Nemelivsky, Yelena.

In: Angle Orthodontist, Vol. 77, No. 1, 01.2007, p. 129-134.

Research output: Contribution to journalArticle

Teixeira, Cristina ; Costas, A. P P ; Nemelivsky, Yelena. / Apoptosis of growth plate chondrocytes occurs through a mitochondrial pathway. In: Angle Orthodontist. 2007 ; Vol. 77, No. 1. pp. 129-134.
@article{ae07c731b33043cbbb10fa3909654853,
title = "Apoptosis of growth plate chondrocytes occurs through a mitochondrial pathway",
abstract = "Objective: To determine the role of mitochondria in chondrocyte apoptosis induced by inorganic phosphate (Pi). Materials and Methods: Chondrocytes isolated from the growth plates of chick embryo tibia were treated with Pi in serum-free media; chondrocyte viability, mitochondrial membrane potential, cytochrome c release from mitochondria, caspase 3 activity, endonuclease activity, and DNA fragmentation were investigated. Results: Exposure to Pi for 24 hours induced apoptosis in growth plate chondrocytes through a pathway that involved loss of mitochondrial function, release of cytochrome c into the cytoplasm, increases in caspase 3 and endonuclease activities, and fragmentation of DNA. Conclusions: This study suggests that mitochondria are important players in Pi-induced apoptosis.",
keywords = "Apoptosis, Caspases, Chondrocytes, Endonucleases, Growth plate, Inorganic phosphate",
author = "Cristina Teixeira and Costas, {A. P P} and Yelena Nemelivsky",
year = "2007",
month = "1",
doi = "10.2319/062805-210R.1",
language = "English (US)",
volume = "77",
pages = "129--134",
journal = "Angle Orthodontist",
issn = "0003-3219",
publisher = "E H Angle Orthodontists Research & Education Foundation, Inc.",
number = "1",

}

TY - JOUR

T1 - Apoptosis of growth plate chondrocytes occurs through a mitochondrial pathway

AU - Teixeira, Cristina

AU - Costas, A. P P

AU - Nemelivsky, Yelena

PY - 2007/1

Y1 - 2007/1

N2 - Objective: To determine the role of mitochondria in chondrocyte apoptosis induced by inorganic phosphate (Pi). Materials and Methods: Chondrocytes isolated from the growth plates of chick embryo tibia were treated with Pi in serum-free media; chondrocyte viability, mitochondrial membrane potential, cytochrome c release from mitochondria, caspase 3 activity, endonuclease activity, and DNA fragmentation were investigated. Results: Exposure to Pi for 24 hours induced apoptosis in growth plate chondrocytes through a pathway that involved loss of mitochondrial function, release of cytochrome c into the cytoplasm, increases in caspase 3 and endonuclease activities, and fragmentation of DNA. Conclusions: This study suggests that mitochondria are important players in Pi-induced apoptosis.

AB - Objective: To determine the role of mitochondria in chondrocyte apoptosis induced by inorganic phosphate (Pi). Materials and Methods: Chondrocytes isolated from the growth plates of chick embryo tibia were treated with Pi in serum-free media; chondrocyte viability, mitochondrial membrane potential, cytochrome c release from mitochondria, caspase 3 activity, endonuclease activity, and DNA fragmentation were investigated. Results: Exposure to Pi for 24 hours induced apoptosis in growth plate chondrocytes through a pathway that involved loss of mitochondrial function, release of cytochrome c into the cytoplasm, increases in caspase 3 and endonuclease activities, and fragmentation of DNA. Conclusions: This study suggests that mitochondria are important players in Pi-induced apoptosis.

KW - Apoptosis

KW - Caspases

KW - Chondrocytes

KW - Endonucleases

KW - Growth plate

KW - Inorganic phosphate

UR - http://www.scopus.com/inward/record.url?scp=33846071583&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846071583&partnerID=8YFLogxK

U2 - 10.2319/062805-210R.1

DO - 10.2319/062805-210R.1

M3 - Article

C2 - 17029540

AN - SCOPUS:33846071583

VL - 77

SP - 129

EP - 134

JO - Angle Orthodontist

JF - Angle Orthodontist

SN - 0003-3219

IS - 1

ER -