Antipeptide antibodies against the 5-HT1A receptor

Efrain C. Azmitia, Ilje Yu, Homayoon M. Akbari, Nancy Kheck, Patricia M. Whitaker-Azmitia, Daniel R. Marshak

Research output: Contribution to journalArticle

Abstract

The availability of the primary amino acid sequence for a large numbers of molecules provides a fruitful opportunity for their cellular localization by utilizing the procedure of antipeptide antibody formation. This procedure permits a synthetic peptide sequence to be attached to a carrier molecule for the purpose of inoculating an animal to raise specific antibodies against the selected protein sequence. In this report we describe a number of steps that can be taken to increase the likelihood that the selected peptide sequence will be specific and antigenic. In addition, we describe how the peptides are synthesized, purified and coupled to keyhold limpet hemocyanin. The preparation of the antibody and its characterization are also presented in this method report. The immunocytochemical staining at both the light and ultrastructural level with serotonin (5-HT1A) receptor antipeptide antibodies is discussed. The advantages and disadvantages of this procedure are summarized.

Original languageEnglish (US)
Pages (from-to)289-298
Number of pages10
JournalJournal of chemical neuroanatomy
Volume5
Issue number4
DOIs
StatePublished - Jan 1 1992

Keywords

  • Immunocytochemistry
  • Serotonin
  • Ultrastructure

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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    Azmitia, E. C., Yu, I., Akbari, H. M., Kheck, N., Whitaker-Azmitia, P. M., & Marshak, D. R. (1992). Antipeptide antibodies against the 5-HT1A receptor. Journal of chemical neuroanatomy, 5(4), 289-298. https://doi.org/10.1016/0891-0618(92)90016-J