Abstract
The availability of the primary amino acid sequence for a large numbers of molecules provides a fruitful opportunity for their cellular localization by utilizing the procedure of antipeptide antibody formation. This procedure permits a synthetic peptide sequence to be attached to a carrier molecule for the purpose of inoculating an animal to raise specific antibodies against the selected protein sequence. In this report we describe a number of steps that can be taken to increase the likelihood that the selected peptide sequence will be specific and antigenic. In addition, we describe how the peptides are synthesized, purified and coupled to keyhold limpet hemocyanin. The preparation of the antibody and its characterization are also presented in this method report. The immunocytochemical staining at both the light and ultrastructural level with serotonin (5-HT1A) receptor antipeptide antibodies is discussed. The advantages and disadvantages of this procedure are summarized.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 289-298 |
| Number of pages | 10 |
| Journal | Journal of Chemical Neuroanatomy |
| Volume | 5 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1992 |
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Keywords
- Immunocytochemistry
- Serotonin
- Ultrastructure
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
Cite this
Antipeptide antibodies against the 5-HT1A receptor. / Azmitia, Efrain C.; Yu, Ilje; Akbari, Homayoon M.; Kheck, Nancy; Whitaker-Azmitia, Patricia M.; Marshak, Daniel R.
In: Journal of Chemical Neuroanatomy, Vol. 5, No. 4, 1992, p. 289-298.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antipeptide antibodies against the 5-HT1A receptor
AU - Azmitia, Efrain C.
AU - Yu, Ilje
AU - Akbari, Homayoon M.
AU - Kheck, Nancy
AU - Whitaker-Azmitia, Patricia M.
AU - Marshak, Daniel R.
PY - 1992
Y1 - 1992
N2 - The availability of the primary amino acid sequence for a large numbers of molecules provides a fruitful opportunity for their cellular localization by utilizing the procedure of antipeptide antibody formation. This procedure permits a synthetic peptide sequence to be attached to a carrier molecule for the purpose of inoculating an animal to raise specific antibodies against the selected protein sequence. In this report we describe a number of steps that can be taken to increase the likelihood that the selected peptide sequence will be specific and antigenic. In addition, we describe how the peptides are synthesized, purified and coupled to keyhold limpet hemocyanin. The preparation of the antibody and its characterization are also presented in this method report. The immunocytochemical staining at both the light and ultrastructural level with serotonin (5-HT1A) receptor antipeptide antibodies is discussed. The advantages and disadvantages of this procedure are summarized.
AB - The availability of the primary amino acid sequence for a large numbers of molecules provides a fruitful opportunity for their cellular localization by utilizing the procedure of antipeptide antibody formation. This procedure permits a synthetic peptide sequence to be attached to a carrier molecule for the purpose of inoculating an animal to raise specific antibodies against the selected protein sequence. In this report we describe a number of steps that can be taken to increase the likelihood that the selected peptide sequence will be specific and antigenic. In addition, we describe how the peptides are synthesized, purified and coupled to keyhold limpet hemocyanin. The preparation of the antibody and its characterization are also presented in this method report. The immunocytochemical staining at both the light and ultrastructural level with serotonin (5-HT1A) receptor antipeptide antibodies is discussed. The advantages and disadvantages of this procedure are summarized.
KW - Immunocytochemistry
KW - Serotonin
KW - Ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=0026690244&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026690244&partnerID=8YFLogxK
U2 - 10.1016/0891-0618(92)90016-J
DO - 10.1016/0891-0618(92)90016-J
M3 - Article
C2 - 1524716
AN - SCOPUS:0026690244
VL - 5
SP - 289
EP - 298
JO - Journal of Chemical Neuroanatomy
JF - Journal of Chemical Neuroanatomy
SN - 0891-0618
IS - 4
ER -