Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat

Yiqun Deng, Jie Liu, Qi Zheng, David Eliezer, Neville R. Kallenbach, Min Lu

Research output: Contribution to journalArticle

Abstract

Coiled-coil sequences in proteins commonly share a seven-amino acid repeat with nonpolar side chains at the first (a) and fourth (d) positions. We investigate here the role of a 3-3-1 hydrophobic repeat containing nonpolar amino acids at the a, d, and g positions in determining the structures of coiled coils using mutants of the GCN4 leucine zipper dimerization domain. When three charged residues at the g positions in the parental sequence are replaced by nonpolar alanine or valine side chains, stable four-helix structures result. The X-ray crystal structures of the tetramers reveal antiparallel, four-stranded coiled coils in which the a, d, and g side chains interlock in a combination of knobs-into-knobs and knobs-into-holes packing. Interfacial interactions in a coiled coil can therefore be prescribed by hydrophobic-polar patterns beyond the canonical 3-4 heptad repeat. The results suggest that the conserved, charged residues at the g positions in the GCN4 leucine zipper can impart a negative design element to disfavor thermodynamically more stable, antiparallel tetramers.

Original languageEnglish (US)
Pages (from-to)247-255
Number of pages9
JournalStructure
Volume14
Issue number2
DOIs
StatePublished - Feb 2006

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Leucine Zippers
Amino Acids
Valine
Dimerization
Alanine
X-Rays
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Deng, Y., Liu, J., Zheng, Q., Eliezer, D., Kallenbach, N. R., & Lu, M. (2006). Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat. Structure, 14(2), 247-255. https://doi.org/10.1016/j.str.2005.10.010

Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat. / Deng, Yiqun; Liu, Jie; Zheng, Qi; Eliezer, David; Kallenbach, Neville R.; Lu, Min.

In: Structure, Vol. 14, No. 2, 02.2006, p. 247-255.

Research output: Contribution to journalArticle

Deng, Y, Liu, J, Zheng, Q, Eliezer, D, Kallenbach, NR & Lu, M 2006, 'Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat', Structure, vol. 14, no. 2, pp. 247-255. https://doi.org/10.1016/j.str.2005.10.010
Deng, Yiqun ; Liu, Jie ; Zheng, Qi ; Eliezer, David ; Kallenbach, Neville R. ; Lu, Min. / Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat. In: Structure. 2006 ; Vol. 14, No. 2. pp. 247-255.
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