Analysis of osteoblast mineral deposits on orthopaedic/dental implant metals

Matthew W. Squire, John Ricci, Rena Bizios

Research output: Contribution to journalArticle

Abstract

Neonatal rat calvarial osteoblasts were cultured on Ti-6Al-4V, Co-Cr-Mo alloy, 316L stainless steel and polystyrene (reference substrate) in the presence of ascorbic acid and 10 mM β-glycerophosphate for 16, 17, 18, 19, 20, 21, 24 and 28 d. Scanning electron microscopy examination revealed that osteoblasts cultured on these orthopaedic/dental implant metals synthesized and deposited an extracellular matrix containing collagenous and non-collagenous components, as well as mineral nodules of various morphologies. Energy dispersive spectrometry revealed that the mineral deposits consisted of three distinct chemical compositions: calcium phosphate, calcium-sulphur-phosphorus, and calcium only. Backscattered electron imaging demonstrated that both the calcium phosphate and calcium-only deposits were electron dense, while the calcium-sulphur-phosphorus deposits were electron translucent. X-ray diffraction analysis indicated that the bulk of the osteoblast mineral deposits was amorphous hydroxyapatite; in addition, electron diffraction analysis revealed small regions of crystalline hydroxyapatite.

Original languageEnglish (US)
Pages (from-to)725-733
Number of pages9
JournalBiomaterials
Volume17
Issue number7
DOIs
StatePublished - Apr 1996

Fingerprint

Dental prostheses
Mineral resources
Dental Implants
Osteoblasts
Orthopedics
Minerals
Calcium
Metals
Electrons
Calcium phosphate
Durapatite
Hydroxyapatite
Sulfur
Phosphorus
Deposits
Glycerophosphates
Ascorbic acid
Polystyrenes
Stainless Steel
Alloy steel

Keywords

  • Calcium phosphates
  • Mineralization
  • Osteoblasts

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Analysis of osteoblast mineral deposits on orthopaedic/dental implant metals. / Squire, Matthew W.; Ricci, John; Bizios, Rena.

In: Biomaterials, Vol. 17, No. 7, 04.1996, p. 725-733.

Research output: Contribution to journalArticle

Squire, Matthew W. ; Ricci, John ; Bizios, Rena. / Analysis of osteoblast mineral deposits on orthopaedic/dental implant metals. In: Biomaterials. 1996 ; Vol. 17, No. 7. pp. 725-733.
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