Analysis of a human fungiform papillae cDNA library and identification of taste-related genes

Olivier Rossier, Jie Cao, Taufiqul Huque, Andrew Spielman, Roy S. Feldman, Juan F. Medrano, Joseph G. Brand, Johannes le Coutre

Research output: Contribution to journalArticle

Abstract

Various genes related to early events in human gustation have recently been discovered, yet a thorough understanding of taste transduction is hampered by gaps in our knowledge of the signaling chain. As a first step toward gaining additional insight, the expression specificity of genes in human taste tissue needs to be determined. To this end, a fungiform papillae cDNA library has been generated and analyzed. For validation of the library, taste-related gene probes were used to detect known molecules. Subsequently, DNA sequence analysis was performed to identify further candidates. Of 987 clones sequenced, clustering results in 288 contigs. Comparison of these contigs with genomic databases reveals that 207 contigs (71.9%) match known genes, 16 (5.6%) match hypothetical genes, eight (2.8%) match repetitive sequences and 57 (19.8%) have no or low similarity to annotated genes. The results indicate that despite a high level of redundancy, this human fungiform cDNA library contains specific taste markers and is valuable for investigation of both known and novel taste-related genes.

Original languageEnglish (US)
Pages (from-to)13-23
Number of pages11
JournalChemical Senses
Volume29
Issue number1
DOIs
StatePublished - Jan 2004

Fingerprint

Gene Library
cDNA libraries
Genes
genes
Nucleic Acid Repetitive Sequences
repetitive sequences
DNA Sequence Analysis
Libraries
Cluster Analysis
Clone Cells
Databases
sequence analysis
Gene Expression
clones
nucleotide sequences

Keywords

  • cDNA library
  • Gene expression
  • Human fungiform papillae
  • Signal transduction
  • Taste marker
  • Tastomics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Neuroscience(all)
  • Physiology
  • Physiology (medical)
  • Behavioral Neuroscience

Cite this

Rossier, O., Cao, J., Huque, T., Spielman, A., Feldman, R. S., Medrano, J. F., ... le Coutre, J. (2004). Analysis of a human fungiform papillae cDNA library and identification of taste-related genes. Chemical Senses, 29(1), 13-23. https://doi.org/10.1093/chemse/bjh002

Analysis of a human fungiform papillae cDNA library and identification of taste-related genes. / Rossier, Olivier; Cao, Jie; Huque, Taufiqul; Spielman, Andrew; Feldman, Roy S.; Medrano, Juan F.; Brand, Joseph G.; le Coutre, Johannes.

In: Chemical Senses, Vol. 29, No. 1, 01.2004, p. 13-23.

Research output: Contribution to journalArticle

Rossier, O, Cao, J, Huque, T, Spielman, A, Feldman, RS, Medrano, JF, Brand, JG & le Coutre, J 2004, 'Analysis of a human fungiform papillae cDNA library and identification of taste-related genes', Chemical Senses, vol. 29, no. 1, pp. 13-23. https://doi.org/10.1093/chemse/bjh002
Rossier, Olivier ; Cao, Jie ; Huque, Taufiqul ; Spielman, Andrew ; Feldman, Roy S. ; Medrano, Juan F. ; Brand, Joseph G. ; le Coutre, Johannes. / Analysis of a human fungiform papillae cDNA library and identification of taste-related genes. In: Chemical Senses. 2004 ; Vol. 29, No. 1. pp. 13-23.
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