An RNA topoisomerase

Hui Wang, Russell J. Di Gate, Nadrian Seeman

Research output: Contribution to journalArticle

Abstract

A synthetic strand of RNA has been designed so that it can adopt two different topological states (a circle and a trefoil knot) when ligated into a cyclic molecule. The RNA knot and circle have been characterized by their behavior in gel electrophoresis and sedimentation experiments. This system allows one to assay for the existence of an RNA topoisomerase, because the two RNA molecules can be interconverted only by a strand passage event. We find that the interconversion of these two species can be catalyzed by Escherichia coli DNA topoisomerase III, indicating that this enzyme can act as an RNA topoisomerase. The conversion of circles to knots is accompanied by a small amount of RNA catenane generation. These findings suggest that strand passage must be considered a potential component of the folding and modification of RNA structures.

Original languageEnglish (US)
Pages (from-to)9477-9482
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number18
DOIs
StatePublished - Sep 3 1996

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RNA
RNA Folding
Electrophoresis
Gels
Escherichia coli
Enzymes

Keywords

  • molecular topology
  • RNA knot
  • strand passage

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

An RNA topoisomerase. / Wang, Hui; Di Gate, Russell J.; Seeman, Nadrian.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 18, 03.09.1996, p. 9477-9482.

Research output: Contribution to journalArticle

Wang, Hui ; Di Gate, Russell J. ; Seeman, Nadrian. / An RNA topoisomerase. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 18. pp. 9477-9482.
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