An oligomeric C-RING nacre protein influences prenucleation events and organizes mineral nanoparticles

Iva Perovic, Andreas Verch, Eric P. Chang, Ashit Rao, Helmut Cölfen, Roland Kröger, John Evans

Research output: Contribution to journalArticle

Abstract

The mollusk shell nacre layer integrates mineral phases with macromolecular components such as intracrystalline proteins. However, the roles performed by intracrystalline proteins in calcium carbonate nucleation and subsequent postnucleation events (e.g., organization of mineral deposits) in the nacre layer are not known. We find that AP7, a nacre intracrystalline C-RING protein, self-assembles to form amorphous protein oligomers and films on mica that further assemble into larger aggregates or phases in the presence of Ca2+. Using solution nuclear magnetic resonance spectroscopy, we determine that the protein assemblies are stabilized by interdomain interactions involving the aggregation-prone T31-N66 C-terminal C-RING domain but are destabilized by the labile nature of the intrinsically disordered D1-T19 AA N-terminal sequence. Thus, the dynamic, amorphous nature of the AP7 assemblies can be traced to the molecular behavior of the N-terminal sequence. Using potentiometric methods, we observe that AP7 protein phases prolong the time interval for prenucleation cluster formation but neither stabilize nor destabilize ACC clusters. Time-resolved flow cell scanning transmission electron microscopy mineralization studies confirm that AP7 protein phases delay the onset of nucleation and assemble and organize mineral nanoparticles into ring-shaped branching clusters in solution. These phenomena are not observed in protein-deficient assays. We conclude that C-RING AP7 protein phases modulate the time period for early events in nucleation and form strategic associations with forming mineral nanoparticles that lead to mineral organization.

Original languageEnglish (US)
Pages (from-to)7259-7268
Number of pages10
JournalBiochemistry
Volume53
Issue number46
DOIs
StatePublished - Nov 25 2014

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Nacre
Nanoparticles
Minerals
Proteins
Nucleation
Scanning Transmission Electron Microscopy
Calcium Carbonate
Mollusca
Mineral resources
Protein C
Oligomers
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Assays
Agglomeration

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)

Cite this

An oligomeric C-RING nacre protein influences prenucleation events and organizes mineral nanoparticles. / Perovic, Iva; Verch, Andreas; Chang, Eric P.; Rao, Ashit; Cölfen, Helmut; Kröger, Roland; Evans, John.

In: Biochemistry, Vol. 53, No. 46, 25.11.2014, p. 7259-7268.

Research output: Contribution to journalArticle

Perovic, I, Verch, A, Chang, EP, Rao, A, Cölfen, H, Kröger, R & Evans, J 2014, 'An oligomeric C-RING nacre protein influences prenucleation events and organizes mineral nanoparticles', Biochemistry, vol. 53, no. 46, pp. 7259-7268. https://doi.org/10.1021/bi5008854
Perovic I, Verch A, Chang EP, Rao A, Cölfen H, Kröger R et al. An oligomeric C-RING nacre protein influences prenucleation events and organizes mineral nanoparticles. Biochemistry. 2014 Nov 25;53(46):7259-7268. https://doi.org/10.1021/bi5008854
Perovic, Iva ; Verch, Andreas ; Chang, Eric P. ; Rao, Ashit ; Cölfen, Helmut ; Kröger, Roland ; Evans, John. / An oligomeric C-RING nacre protein influences prenucleation events and organizes mineral nanoparticles. In: Biochemistry. 2014 ; Vol. 53, No. 46. pp. 7259-7268.
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