An integrated microfluidic platform for in-situ cellular cytokine secretion immunophenotyping

Nien Tsu Huang, Weiqiang Chen, Bo Ram Oh, Jianping Fu, Katsuo Kurabayashi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Rapid, quantitative detection of cell-secreted biomarker proteins with a low sample volume holds great promise to advance cellular immunophenotyping techniques for diagnosis and treatment of infectious diseases. Here we successfully developed an immunophenotyping assay with THP-1 human moncytic leukemia cells using a highly integrated microfluidic platform incorporating a no-wash bead-based chemiluminescence immunodetection scheme. Our study achieved ultra-sensitive cellular immunophenotyping with 20-fold fewer cells and 7 times faster than the conventional enzyme-linked immunosorbent assay (ELISA). Our strategy of monitoring immune cell functions could impact medical treatments of infectious diseases by enabling a sample-efficient cellular immunophenotyping analysis.

Original languageEnglish (US)
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages989-991
Number of pages3
ISBN (Print)9780979806452
StatePublished - Jan 1 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

    Fingerprint

Keywords

  • Cellular immunophenotyping
  • Chemiluminescence
  • Microfluidics
  • Optics

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Huang, N. T., Chen, W., Oh, B. R., Fu, J., & Kurabayashi, K. (2012). An integrated microfluidic platform for in-situ cellular cytokine secretion immunophenotyping. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 989-991). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.