An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation

Jingyi Zhu, Jiacheng He, Michael Verano, Ayoola T. Brimmo, Ayoub Glia, Mohammad Qasaimeh, Pengyu Chen, Jose O. Aleman, Weiqiang Chen

Research output: Contribution to journalArticle

Abstract

Although many advanced biosensing techniques have been proposed for cytokine profiling, there are no clinically available methods that integrate high-resolution immune cell monitoring and in situ multiplexed cytokine detection together in a biomimetic tissue microenvironment. The primary challenge arises due to the lack of suitable label-free sensing techniques and difficulty for sensor integration. In this work, we demonstrated a novel integration of a localized-surface plasmon resonance (LSPR)-based biosensor with a biomimetic microfluidic 'adipose-tissue-on-chip' platform for an in situ label-free, high-throughput and multiplexed cytokine secretion analysis of obese adipose tissue. Using our established adipose-tissue-on-chip platform, we were able to monitor the adipose tissue initiation, differentiation, and maturation and simulate the hallmark formation of crown-like structures (CLSs) during pro-inflammatory stimulation. With integrated antibody-conjugated LSPR barcode sensor arrays, our platform enables simultaneous multiplexed measurements of pro-inflammatory (IL-6 and TNF-α) and anti-inflammatory (IL-10 and IL-4) cytokines secreted by the adipocytes and macrophages. As a result, our adipose-tissue-on-chip platform is capable of identifying stage-specific cytokine secretion profiles from a complex milieu during obesity progression, highlighting its potential as a high-throughput preclinical readout for personalized obesity treatment strategies.

Original languageEnglish (US)
Pages (from-to)3550-3560
Number of pages11
JournalLab on a Chip
Volume18
Issue number23
DOIs
StatePublished - Dec 7 2018

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Adipose Tissue
Obesity
Tissue
Cytokines
Inflammation
Biomimetics
Surface Plasmon Resonance
Biosensing Techniques
Surface plasmon resonance
Labels
Immunologic Monitoring
Throughput
Microfluidics
Crowns
Adipocytes
Macrophages
Interleukin-4
Interleukin-10
Sensor arrays
Interleukin-6

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation. / Zhu, Jingyi; He, Jiacheng; Verano, Michael; Brimmo, Ayoola T.; Glia, Ayoub; Qasaimeh, Mohammad; Chen, Pengyu; Aleman, Jose O.; Chen, Weiqiang.

In: Lab on a Chip, Vol. 18, No. 23, 07.12.2018, p. 3550-3560.

Research output: Contribution to journalArticle

Zhu, J, He, J, Verano, M, Brimmo, AT, Glia, A, Qasaimeh, M, Chen, P, Aleman, JO & Chen, W 2018, 'An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation', Lab on a Chip, vol. 18, no. 23, pp. 3550-3560. https://doi.org/10.1039/c8lc00605a
Zhu, Jingyi ; He, Jiacheng ; Verano, Michael ; Brimmo, Ayoola T. ; Glia, Ayoub ; Qasaimeh, Mohammad ; Chen, Pengyu ; Aleman, Jose O. ; Chen, Weiqiang. / An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation. In: Lab on a Chip. 2018 ; Vol. 18, No. 23. pp. 3550-3560.
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