An improved collagen scaffold for skeletal regeneration

Serafim M. Oliveira, Rushali A. Ringshia, Racquel Z. Legeros, Elizabeth Clark, Michael J. Yost, Louis Terracio, Cristina Teixeira

Research output: Contribution to journalArticle

Abstract

Bone repair and regeneration is one of the most extensively studied areas in the field of tissue engineering. All of the current tissue engineering approaches to create bone focus on intramembranous ossification, ignoring the other mechanism of bone formation, endochondral ossification. We propose to create a transient cartilage template in vitro, which could serve as an intermediate for bone formation by the endochondral mechanism once implanted in vivo. The goals of the study are (1) to prepare and characterize type I collagen sponges as a scaffold for the cartilage template, and (2) to establish a method of culturing chondrocytes in type I collagen sponges and induce cell maturation. Collagen sponges were generated from a 1% solution of type I collagen using a freeze/dry technique followed by UV light crosslinking. Chondrocytes isolated from two locations in chick embryo sterna were cultured in these sponges and treated with retinoic acid to induce chondrocyte maturation and extracellular matrix deposition. Material strength testing as well as microscopic and biochemical analyzes were conducted to evaluate the properties of sponges and cell behavior during the culture period. We found that our collagen sponges presented improved stiffness and supported chondrocyte attachment and proliferation. Cells underwent maturation, depositing an abundant extracellular matrix throughout the scaffold, expressing high levels of type X collagen, type I collagen and alkaline phosphatase. These results demonstrate that we have created a transient cartilage template with potential to direct endochondral bone formation after implantation.

Original languageEnglish (US)
Pages (from-to)371-379
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume94
Issue number2
DOIs
StatePublished - 2010

Fingerprint

Porifera
Scaffolds (biology)
Collagen
Scaffolds
Regeneration
Bone
Collagen Type I
Osteogenesis
Chondrocytes
Cartilage
Tissue engineering
Tissue Engineering
Extracellular Matrix
Materials Testing
Sternum
Tretinoin
Bone Regeneration
Ultraviolet radiation
Crosslinking
Alkaline Phosphatase

Keywords

  • Alkaline phosphatase
  • Chondrocytes
  • Endochondral ossification
  • Tissue engineering
  • Type I collagen
  • Type X collagen

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys
  • Medicine(all)

Cite this

An improved collagen scaffold for skeletal regeneration. / Oliveira, Serafim M.; Ringshia, Rushali A.; Legeros, Racquel Z.; Clark, Elizabeth; Yost, Michael J.; Terracio, Louis; Teixeira, Cristina.

In: Journal of Biomedical Materials Research - Part A, Vol. 94, No. 2, 2010, p. 371-379.

Research output: Contribution to journalArticle

Oliveira, Serafim M. ; Ringshia, Rushali A. ; Legeros, Racquel Z. ; Clark, Elizabeth ; Yost, Michael J. ; Terracio, Louis ; Teixeira, Cristina. / An improved collagen scaffold for skeletal regeneration. In: Journal of Biomedical Materials Research - Part A. 2010 ; Vol. 94, No. 2. pp. 371-379.
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