An immobile nucleic acid junction constructed from oligonucleotides

N. R. Kallenbach, R. I. Ma, Nadrian Seeman

Research output: Contribution to journalArticle

Abstract

Bases-paired DNA duplexes involving oligonucleotide model systems have provided the major source of detailed structural and dynamic information abour double helical structure. Triple- and quadruple-branched 'junction' structure of DNA have a transient existence as intermediates in the replication or recombination of DNA molecules while cruciforms may be inducible by negatively supercoiling closed circular DNA. However, it has not been possible to investigate these forms structurally at high resolution in short-chain molecules, where the junction will yield a significant component of the signal, because these naturally occurring intermediates are inherently unstable, due to internal sequence symmetry, which permits their resolution to double helices, via branchpoint migration. We have recently proposed that migration can be eliminated to yield immobile junctions from oligonucleotides by combining sequence symmetry constraints with equilibrium calculations. We present here electrophoretic and UV optical absorbance experiments which indicate that four hexadecadeoxynucleotides indeed do form a stable tetrameric junction complex in solution.

Original languageEnglish (US)
Pages (from-to)829-831
Number of pages3
JournalNature
Volume305
Issue number5937
StatePublished - 1983

Fingerprint

oligonucleotides
nucleic acids
deoxyribonucleic acid
symmetry
helices
molecules
high resolution

ASJC Scopus subject areas

  • General

Cite this

Kallenbach, N. R., Ma, R. I., & Seeman, N. (1983). An immobile nucleic acid junction constructed from oligonucleotides. Nature, 305(5937), 829-831.

An immobile nucleic acid junction constructed from oligonucleotides. / Kallenbach, N. R.; Ma, R. I.; Seeman, Nadrian.

In: Nature, Vol. 305, No. 5937, 1983, p. 829-831.

Research output: Contribution to journalArticle

Kallenbach, NR, Ma, RI & Seeman, N 1983, 'An immobile nucleic acid junction constructed from oligonucleotides', Nature, vol. 305, no. 5937, pp. 829-831.
Kallenbach, N. R. ; Ma, R. I. ; Seeman, Nadrian. / An immobile nucleic acid junction constructed from oligonucleotides. In: Nature. 1983 ; Vol. 305, No. 5937. pp. 829-831.
@article{0276c08e7d934a4f942cb4e16fed554c,
title = "An immobile nucleic acid junction constructed from oligonucleotides",
abstract = "Bases-paired DNA duplexes involving oligonucleotide model systems have provided the major source of detailed structural and dynamic information abour double helical structure. Triple- and quadruple-branched 'junction' structure of DNA have a transient existence as intermediates in the replication or recombination of DNA molecules while cruciforms may be inducible by negatively supercoiling closed circular DNA. However, it has not been possible to investigate these forms structurally at high resolution in short-chain molecules, where the junction will yield a significant component of the signal, because these naturally occurring intermediates are inherently unstable, due to internal sequence symmetry, which permits their resolution to double helices, via branchpoint migration. We have recently proposed that migration can be eliminated to yield immobile junctions from oligonucleotides by combining sequence symmetry constraints with equilibrium calculations. We present here electrophoretic and UV optical absorbance experiments which indicate that four hexadecadeoxynucleotides indeed do form a stable tetrameric junction complex in solution.",
author = "Kallenbach, {N. R.} and Ma, {R. I.} and Nadrian Seeman",
year = "1983",
language = "English (US)",
volume = "305",
pages = "829--831",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Publishing Group",
number = "5937",

}

TY - JOUR

T1 - An immobile nucleic acid junction constructed from oligonucleotides

AU - Kallenbach, N. R.

AU - Ma, R. I.

AU - Seeman, Nadrian

PY - 1983

Y1 - 1983

N2 - Bases-paired DNA duplexes involving oligonucleotide model systems have provided the major source of detailed structural and dynamic information abour double helical structure. Triple- and quadruple-branched 'junction' structure of DNA have a transient existence as intermediates in the replication or recombination of DNA molecules while cruciforms may be inducible by negatively supercoiling closed circular DNA. However, it has not been possible to investigate these forms structurally at high resolution in short-chain molecules, where the junction will yield a significant component of the signal, because these naturally occurring intermediates are inherently unstable, due to internal sequence symmetry, which permits their resolution to double helices, via branchpoint migration. We have recently proposed that migration can be eliminated to yield immobile junctions from oligonucleotides by combining sequence symmetry constraints with equilibrium calculations. We present here electrophoretic and UV optical absorbance experiments which indicate that four hexadecadeoxynucleotides indeed do form a stable tetrameric junction complex in solution.

AB - Bases-paired DNA duplexes involving oligonucleotide model systems have provided the major source of detailed structural and dynamic information abour double helical structure. Triple- and quadruple-branched 'junction' structure of DNA have a transient existence as intermediates in the replication or recombination of DNA molecules while cruciforms may be inducible by negatively supercoiling closed circular DNA. However, it has not been possible to investigate these forms structurally at high resolution in short-chain molecules, where the junction will yield a significant component of the signal, because these naturally occurring intermediates are inherently unstable, due to internal sequence symmetry, which permits their resolution to double helices, via branchpoint migration. We have recently proposed that migration can be eliminated to yield immobile junctions from oligonucleotides by combining sequence symmetry constraints with equilibrium calculations. We present here electrophoretic and UV optical absorbance experiments which indicate that four hexadecadeoxynucleotides indeed do form a stable tetrameric junction complex in solution.

UR - http://www.scopus.com/inward/record.url?scp=0020545065&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020545065&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0020545065

VL - 305

SP - 829

EP - 831

JO - Nature Cell Biology

JF - Nature Cell Biology

SN - 1465-7392

IS - 5937

ER -