An FGF-driven feed-forward circuit patterns the cardiopharyngeal mesoderm in space and time

Florian Razy-Krajka, Basile Gravez, Nicole Kaplan, Claudia Racioppi, Wei Wang, Lionel Christiaen

Research output: Contribution to journalArticle

Abstract

In embryos, multipotent progenitors divide to produce distinct progeny and express their full potential. In vertebrates, multipotent cardiopharyngeal progenitors produce second-heartfield- derived cardiomyocytes, and branchiomeric skeletal head muscles. However, the mechanisms underlying these early fate choices remain largely elusive. The tunicate Ciona emerged as an attractive model to study early cardiopharyngeal development at high resolution: through two asymmetric and oriented divisions, defined cardiopharyngeal progenitors produce distinct first and second heart precursors, and pharyngeal muscle (aka atrial siphon muscle, ASM) precursors. Here, we demonstrate that differential FGF-MAPK signaling distinguishes between heart and ASM precursors. We characterize a feed-forward circuit that promotes the successive activations of essential ASM determinants, Hand-related, Tbx1/10 and Ebf. Finally, we show that coupling FGFMAPK restriction and cardiopharyngeal network deployment with cell divisions defines the timing of gene expression and permits the emergence of diverse cell types from multipotent progenitors.

Original languageEnglish (US)
Article numbere29656
JournaleLife
Volume7
DOIs
StatePublished - Feb 6 2018

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Mesoderm
Muscle
Siphons
Muscles
Networks (circuits)
Pharyngeal Muscles
Urochordata
Cardiac Myocytes
Cell Division
Vertebrates
Myocardium
Skeletal Muscle
Stem Cells
Embryonic Structures
Hand
Head
Gene Expression
Gene expression
Chemical activation
Cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

An FGF-driven feed-forward circuit patterns the cardiopharyngeal mesoderm in space and time. / Razy-Krajka, Florian; Gravez, Basile; Kaplan, Nicole; Racioppi, Claudia; Wang, Wei; Christiaen, Lionel.

In: eLife, Vol. 7, e29656, 06.02.2018.

Research output: Contribution to journalArticle

Razy-Krajka, Florian ; Gravez, Basile ; Kaplan, Nicole ; Racioppi, Claudia ; Wang, Wei ; Christiaen, Lionel. / An FGF-driven feed-forward circuit patterns the cardiopharyngeal mesoderm in space and time. In: eLife. 2018 ; Vol. 7.
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