An electrochemiluminescence assay for gp340 (DMBT1)

Cheryl Barber, Drew Weissman, Kurt Barnhart, Minal Dalvi, William R. Abrams, Daniel Malamud

Research output: Contribution to journalArticle

Abstract

Gp340 is a member of the scavenger receptor cysteine-rich family of innate immune molecules and also functions as a tumor suppressor. This study describes a picogram-level assay using electrochemiluminescence technology on the MesoScale Discovery platform. Antibodies were evaluated and the best pair was used to assay whole-mouth stimulated saliva and cervical/vaginal lavage. The assay was tested using specimens obtained from healthy volunteers to determine if gp340 concentration in saliva correlates with levels in vaginal lavage fluid. Interestingly, no correlation was determined between gp340 content in these two fluids.

Original languageEnglish (US)
Pages (from-to)78-80
Number of pages3
JournalAnalytical Biochemistry
Volume440
Issue number1
DOIs
StatePublished - 2013

Fingerprint

Vaginal Douching
Saliva
Assays
Scavenger Receptors
Cysteine
Mouth
Healthy Volunteers
Fluids
Technology
Antibodies
Tumors
Neoplasms
Molecules

Keywords

  • Cervical vaginal lavage
  • MesoScale Discovery
  • Salivary agglutinin
  • Scavenger receptor cysteine-rich
  • Whole-mouth stimulated saliva

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Barber, C., Weissman, D., Barnhart, K., Dalvi, M., Abrams, W. R., & Malamud, D. (2013). An electrochemiluminescence assay for gp340 (DMBT1). Analytical Biochemistry, 440(1), 78-80. https://doi.org/10.1016/j.ab.2013.05.015

An electrochemiluminescence assay for gp340 (DMBT1). / Barber, Cheryl; Weissman, Drew; Barnhart, Kurt; Dalvi, Minal; Abrams, William R.; Malamud, Daniel.

In: Analytical Biochemistry, Vol. 440, No. 1, 2013, p. 78-80.

Research output: Contribution to journalArticle

Barber, C, Weissman, D, Barnhart, K, Dalvi, M, Abrams, WR & Malamud, D 2013, 'An electrochemiluminescence assay for gp340 (DMBT1)', Analytical Biochemistry, vol. 440, no. 1, pp. 78-80. https://doi.org/10.1016/j.ab.2013.05.015
Barber, Cheryl ; Weissman, Drew ; Barnhart, Kurt ; Dalvi, Minal ; Abrams, William R. ; Malamud, Daniel. / An electrochemiluminescence assay for gp340 (DMBT1). In: Analytical Biochemistry. 2013 ; Vol. 440, No. 1. pp. 78-80.
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