An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae

Vanessa Borges, Duncan Smith, Iestyn Whitehouse, Frank Uhlmann

Research output: Contribution to journalArticle

Abstract

Cohesion between sister chromatids, mediated by the chromosomal cohesin complex, is a prerequisite for their alignment on the spindle apparatus and segregation in mitosis. Budding yeast cohesin first associates with chromosomes in G1. Then, during DNA replication in S-phase, the replication fork-associated acetyltransferase Eco1 acetylates the cohesin subunit Smc3 to make cohesin's DNA binding resistant to destabilization by the Wapl protein. Whether stabilization of cohesin molecules that happen to link sister chromatids is sufficient to build sister chromatid cohesion, or whether additional reactions are required to establish these links, is not known. In addition to Eco1, several other factors contribute to cohesion establishment, including Ctf4, Ctf18, Tof1, Csm3, Chl1 and Mrc1, but little is known about their roles. Here, we show that each of these factors facilitates cohesin acetylation. Moreover, the absence of Ctf4 and Chl1, but not of the other factors, causes a synthetic growth defect in cells lacking Eco1. Distinct from acetylation defects, sister chromatid cohesion in ctf4Δ and chl1Δ cells is not improved by removing Wapl. Unlike previously thought, we do not find evidence for a role of Ctf4 and Chl1 in Okazaki fragment processing, or of Okazaki fragment processing in sister chromatid cohesion. Thus, Ctf4 and Chl1 delineate an additional acetylation-independent pathway that might hold important clues as to the mechanism of sister chromatid cohesion establishment.

Original languageEnglish (US)
Pages (from-to)121-134
Number of pages14
JournalChromosoma
Volume122
Issue number1-2
DOIs
StatePublished - Mar 2013

Fingerprint

Chromatids
Saccharomyces cerevisiae
Acetylation
Spindle Apparatus
Acetyltransferases
Saccharomycetales
DNA Replication
S Phase
Mitosis
cohesins
Chromosomes
DNA
Growth
Proteins

Keywords

  • Chl1
  • Cohesin
  • Ctf4
  • Eco1
  • S. cerevisiae
  • Sister chromatid cohesion

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae. / Borges, Vanessa; Smith, Duncan; Whitehouse, Iestyn; Uhlmann, Frank.

In: Chromosoma, Vol. 122, No. 1-2, 03.2013, p. 121-134.

Research output: Contribution to journalArticle

Borges, Vanessa ; Smith, Duncan ; Whitehouse, Iestyn ; Uhlmann, Frank. / An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae. In: Chromosoma. 2013 ; Vol. 122, No. 1-2. pp. 121-134.
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