An acylatable residue of Hedgehog is differentially required in Drosophila and mouse limb development

Jeffrey D. Lee, Petra Kraus, Nicholas Gaiano, Susana Nery, Jhumku Kohtz, Gordon Fishell, Cynthia A. Loomis, Jessica E. Treisman

Research output: Contribution to journalArticle

Abstract

The Drosophila Hedgehog protein and its vertebrate counterpart Sonic hedgehog are required for a wide variety of patterning events throughout development. Hedgehog proteins are secreted from cells and undergo autocatalytic cleavage and cholesterol modification to produce a mature signaling domain. This domain of Sonic hedgehog has recently been shown to acquire an N-terminal acyl group in cell culture. We have investigated the in vivo role that such acylation might play in appendage patterning in mouse and Drosophila; in both species Hedgehog proteins define a posterior domain of the limb or wing. A mutant form of Sonic hedgehog that cannot undergo acylation retains significant ability to repattern the mouse limb. However, the corresponding mutation in Drosophila Hedgehog renders it inactive in vivo, although it is normally processed. Furthermore, overexpression of the mutant form has dominant negative effects on Hedgehog signaling. These data suggest that the importance of the N-terminal cysteine of mature Hedgehog in patterning appendages differs between species.

Original languageEnglish (US)
Pages (from-to)122-136
Number of pages15
JournalDevelopmental Biology
Volume233
Issue number1
DOIs
StatePublished - May 1 2001

Fingerprint

Hedgehogs
Drosophila
Extremities
Hedgehog Proteins
Acylation
Cysteine
Vertebrates
Cell Culture Techniques
Cholesterol
Mutation

Keywords

  • Acylation
  • Evolution
  • Hedgehog
  • Limb
  • Wing

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

An acylatable residue of Hedgehog is differentially required in Drosophila and mouse limb development. / Lee, Jeffrey D.; Kraus, Petra; Gaiano, Nicholas; Nery, Susana; Kohtz, Jhumku; Fishell, Gordon; Loomis, Cynthia A.; Treisman, Jessica E.

In: Developmental Biology, Vol. 233, No. 1, 01.05.2001, p. 122-136.

Research output: Contribution to journalArticle

Lee, JD, Kraus, P, Gaiano, N, Nery, S, Kohtz, J, Fishell, G, Loomis, CA & Treisman, JE 2001, 'An acylatable residue of Hedgehog is differentially required in Drosophila and mouse limb development', Developmental Biology, vol. 233, no. 1, pp. 122-136. https://doi.org/10.1006/dbio.2001.0218
Lee, Jeffrey D. ; Kraus, Petra ; Gaiano, Nicholas ; Nery, Susana ; Kohtz, Jhumku ; Fishell, Gordon ; Loomis, Cynthia A. ; Treisman, Jessica E. / An acylatable residue of Hedgehog is differentially required in Drosophila and mouse limb development. In: Developmental Biology. 2001 ; Vol. 233, No. 1. pp. 122-136.
@article{73ece2b3deee47a69e6819569bf0ff5d,
title = "An acylatable residue of Hedgehog is differentially required in Drosophila and mouse limb development",
abstract = "The Drosophila Hedgehog protein and its vertebrate counterpart Sonic hedgehog are required for a wide variety of patterning events throughout development. Hedgehog proteins are secreted from cells and undergo autocatalytic cleavage and cholesterol modification to produce a mature signaling domain. This domain of Sonic hedgehog has recently been shown to acquire an N-terminal acyl group in cell culture. We have investigated the in vivo role that such acylation might play in appendage patterning in mouse and Drosophila; in both species Hedgehog proteins define a posterior domain of the limb or wing. A mutant form of Sonic hedgehog that cannot undergo acylation retains significant ability to repattern the mouse limb. However, the corresponding mutation in Drosophila Hedgehog renders it inactive in vivo, although it is normally processed. Furthermore, overexpression of the mutant form has dominant negative effects on Hedgehog signaling. These data suggest that the importance of the N-terminal cysteine of mature Hedgehog in patterning appendages differs between species.",
keywords = "Acylation, Evolution, Hedgehog, Limb, Wing",
author = "Lee, {Jeffrey D.} and Petra Kraus and Nicholas Gaiano and Susana Nery and Jhumku Kohtz and Gordon Fishell and Loomis, {Cynthia A.} and Treisman, {Jessica E.}",
year = "2001",
month = "5",
day = "1",
doi = "10.1006/dbio.2001.0218",
language = "English (US)",
volume = "233",
pages = "122--136",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - An acylatable residue of Hedgehog is differentially required in Drosophila and mouse limb development

AU - Lee, Jeffrey D.

AU - Kraus, Petra

AU - Gaiano, Nicholas

AU - Nery, Susana

AU - Kohtz, Jhumku

AU - Fishell, Gordon

AU - Loomis, Cynthia A.

AU - Treisman, Jessica E.

PY - 2001/5/1

Y1 - 2001/5/1

N2 - The Drosophila Hedgehog protein and its vertebrate counterpart Sonic hedgehog are required for a wide variety of patterning events throughout development. Hedgehog proteins are secreted from cells and undergo autocatalytic cleavage and cholesterol modification to produce a mature signaling domain. This domain of Sonic hedgehog has recently been shown to acquire an N-terminal acyl group in cell culture. We have investigated the in vivo role that such acylation might play in appendage patterning in mouse and Drosophila; in both species Hedgehog proteins define a posterior domain of the limb or wing. A mutant form of Sonic hedgehog that cannot undergo acylation retains significant ability to repattern the mouse limb. However, the corresponding mutation in Drosophila Hedgehog renders it inactive in vivo, although it is normally processed. Furthermore, overexpression of the mutant form has dominant negative effects on Hedgehog signaling. These data suggest that the importance of the N-terminal cysteine of mature Hedgehog in patterning appendages differs between species.

AB - The Drosophila Hedgehog protein and its vertebrate counterpart Sonic hedgehog are required for a wide variety of patterning events throughout development. Hedgehog proteins are secreted from cells and undergo autocatalytic cleavage and cholesterol modification to produce a mature signaling domain. This domain of Sonic hedgehog has recently been shown to acquire an N-terminal acyl group in cell culture. We have investigated the in vivo role that such acylation might play in appendage patterning in mouse and Drosophila; in both species Hedgehog proteins define a posterior domain of the limb or wing. A mutant form of Sonic hedgehog that cannot undergo acylation retains significant ability to repattern the mouse limb. However, the corresponding mutation in Drosophila Hedgehog renders it inactive in vivo, although it is normally processed. Furthermore, overexpression of the mutant form has dominant negative effects on Hedgehog signaling. These data suggest that the importance of the N-terminal cysteine of mature Hedgehog in patterning appendages differs between species.

KW - Acylation

KW - Evolution

KW - Hedgehog

KW - Limb

KW - Wing

UR - http://www.scopus.com/inward/record.url?scp=0035337027&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035337027&partnerID=8YFLogxK

U2 - 10.1006/dbio.2001.0218

DO - 10.1006/dbio.2001.0218

M3 - Article

C2 - 11319862

AN - SCOPUS:0035337027

VL - 233

SP - 122

EP - 136

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -