An actin-ribonucleoprotein interaction is involved in transcription by RNA polymerase II

Piergiorgio Percipalle, Nathalie Fomproix, Karin Kylberg, Francesc Miralles, Birgitta Björkroth, Bertil Daneholt, Neus Visa

Research output: Contribution to journalArticle

Abstract

To determine the function of actin in the cell nucleus, we sought to identify nuclear actin-binding proteins in the dipteran Chironomus tentans using DNase I-affinity chromatography. We identified the RNA-binding protein hrp65 as an actin-binding protein and showed that the C-terminal sequence of the hrp65-2 isoform is able to interact directly with actin in vitro. In vivo crosslinking and coimmunoprecipitation experiments indicated that hrp65 and actin are also associated in the living cell. Moreover, in vivo administration of a competing peptide corresponding to the C-terminal sequence of hrp65-2 disrupted the actin-hrp65-2 interaction and caused a specific and drastic reduction of transcription as judged by puff regression and diminished bromo-UTP incorporation. Our results indicate that an actin-based mechanism is implicated in the transcription of most if not all RNA polymerase II genes and suggest that an actin-hrp65-2 interaction is required to maintain the normal transcriptional activity of the cell. Furthermore, immunoelectron microscopy experiments and nuclear run-on assays suggest that the actin-hrp65-2 complex plays a role in transcription elongation.

Original languageEnglish (US)
Pages (from-to)6475-6480
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number11
DOIs
StatePublished - May 27 2003

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Ribonucleoproteins
RNA Polymerase II
Actins
Microfilament Proteins
Chironomidae
Uridine Triphosphate
RNA-Binding Proteins
Immunoelectron Microscopy
Deoxyribonuclease I
Nuclear Proteins
Protein C
Cell Nucleus
Affinity Chromatography
Protein Isoforms
Peptides
Genes

ASJC Scopus subject areas

  • Genetics
  • General

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An actin-ribonucleoprotein interaction is involved in transcription by RNA polymerase II. / Percipalle, Piergiorgio; Fomproix, Nathalie; Kylberg, Karin; Miralles, Francesc; Björkroth, Birgitta; Daneholt, Bertil; Visa, Neus.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 11, 27.05.2003, p. 6475-6480.

Research output: Contribution to journalArticle

Percipalle, Piergiorgio ; Fomproix, Nathalie ; Kylberg, Karin ; Miralles, Francesc ; Björkroth, Birgitta ; Daneholt, Bertil ; Visa, Neus. / An actin-ribonucleoprotein interaction is involved in transcription by RNA polymerase II. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 11. pp. 6475-6480.
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AU - Fomproix, Nathalie

AU - Kylberg, Karin

AU - Miralles, Francesc

AU - Björkroth, Birgitta

AU - Daneholt, Bertil

AU - Visa, Neus

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