An actin-myosin complex on actively transcribing genes

Nathalie Fomproix, Piergiorgio Percipalle

Research output: Contribution to journalArticle

Abstract

Actin and myosin have been individually implicated in different aspects of gene expression. Here, we show in vivo evidence for a specific nucleolar actin-myosin complex physically associated with both the RNA polymerase I holoenzyme and ribosomal genes. We find that this specific actin-myosin complex is functionally coupled to elongating ribosomal RNA transcripts in living cells. From these observations, we conclude that an actin-based myosin motor is associated with transcribing ribosomal genes in the cell nucleus. These results correlate with an involvement of both actin and myosin in regulating mRNA synthesis and suggest that actin-myosin motors may provide a general mechanism to facilitate elongation of RNA transcripts during transcription of both ribosomal genes and protein-coding genes.

Original languageEnglish (US)
Pages (from-to)140-148
Number of pages9
JournalExperimental Cell Research
Volume294
Issue number1
DOIs
StatePublished - Mar 10 2004

Fingerprint

Myosins
Actins
Genes
RNA Polymerase I
Holoenzymes
Ribosomal RNA
Ribosomal Proteins
Cell Nucleus
RNA
Gene Expression
Messenger RNA
Proteins

Keywords

  • Actin-myosin complex
  • Intranuclear motors
  • Ribosomal genes
  • RNA elongation
  • RNA polymerase I
  • Transcription

ASJC Scopus subject areas

  • Cell Biology

Cite this

An actin-myosin complex on actively transcribing genes. / Fomproix, Nathalie; Percipalle, Piergiorgio.

In: Experimental Cell Research, Vol. 294, No. 1, 10.03.2004, p. 140-148.

Research output: Contribution to journalArticle

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