Amygdala kindling alters protein kinase C activity in dentate gyrus

Shu Jen Chen, Manisha A. Desai, Eric Klann, Danny G. Winder, J. David Sweatt, P. Jeffrey Conn

Research output: Contribution to journalArticle

Abstract

Kindling is a use-dependent form of synaptic plasticity and a widely used model of epilepsy. Although kindling has been widely studied, the molecular mechanisms underlying induction of this phenomenon are not well understood. We determined the effect of amygdala kindling on protein kinase C (PKC) activity in various regions of rat brain. Kindling stimulation markedly elevated basal (Ca2+-independent) and Ca2+-stimulated phosphorylation of an endogenous PKC substrate (which we have termed P17) in homogenates of dentate gyrus, assayed 2 h after kindling stimulation. The increase in P17 phosphorylation appeared to be due at least in part to persistent PKC activation, as basal PKC activity assayed in vitro using an exogenous peptide substrate was increased in kindled dentate gyrus 2 h after the last kindling stimulation. A similar increase in basal PKC activity was observed in dentate gyrus 2 h after the first kindling stimulation. These results document a kindling-associated persistent PKC activation and suggest that the increased activity of PKC could play a role in the induction of the kindling effect.

Original languageEnglish (US)
Pages (from-to)1761-1769
Number of pages9
JournalJournal of Neurochemistry
Volume59
Issue number5
StatePublished - Nov 1992

Fingerprint

Dentate Gyrus
Amygdala
Protein Kinase C
Phosphorylation
Chemical activation
Neuronal Plasticity
Substrates
Plasticity
Rats
Epilepsy
Brain
Peptides

Keywords

  • Dentate gyrus
  • Epilepsy model
  • Kindling
  • Protein kinase C activity

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Chen, S. J., Desai, M. A., Klann, E., Winder, D. G., Sweatt, J. D., & Conn, P. J. (1992). Amygdala kindling alters protein kinase C activity in dentate gyrus. Journal of Neurochemistry, 59(5), 1761-1769.

Amygdala kindling alters protein kinase C activity in dentate gyrus. / Chen, Shu Jen; Desai, Manisha A.; Klann, Eric; Winder, Danny G.; Sweatt, J. David; Conn, P. Jeffrey.

In: Journal of Neurochemistry, Vol. 59, No. 5, 11.1992, p. 1761-1769.

Research output: Contribution to journalArticle

Chen, SJ, Desai, MA, Klann, E, Winder, DG, Sweatt, JD & Conn, PJ 1992, 'Amygdala kindling alters protein kinase C activity in dentate gyrus', Journal of Neurochemistry, vol. 59, no. 5, pp. 1761-1769.
Chen SJ, Desai MA, Klann E, Winder DG, Sweatt JD, Conn PJ. Amygdala kindling alters protein kinase C activity in dentate gyrus. Journal of Neurochemistry. 1992 Nov;59(5):1761-1769.
Chen, Shu Jen ; Desai, Manisha A. ; Klann, Eric ; Winder, Danny G. ; Sweatt, J. David ; Conn, P. Jeffrey. / Amygdala kindling alters protein kinase C activity in dentate gyrus. In: Journal of Neurochemistry. 1992 ; Vol. 59, No. 5. pp. 1761-1769.
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