Abstract
Neutrophils (polymorphonuclear leucocytes) are the principal cell of the host defence system. Consequently, if periodontal pathogen-derived substances in the gingival crevice significantly inhibit their function, they could shift the bacterial-host balance in favour of the bacteria. The hypothesis that ammonia can inhibit neutrophil function was tested. Ammonia was specifically selected because periodontal pathogens produce substantial amounts of ammonia. The findings indicated that ammonia can inhibit neutrophil phagocytosis, degranulation and oxygen metabolism. Ammonia decreased the total number of phagocytosing polymorphonuclear neutrophils (66% of control) and also decreased degranulation (61% of control). Ammonia decreased oxygen metabolism of both resting and stimulated neutrophils (33 and 42% of control, respectively). These observations support the hypothesis that ammonia can inhibit the function of polymorphonuclear leucocytes. They suggest that the presence of ammonia in the gingival crevice may increase the risk of development of periodonal disease.
| Original language | English (US) |
|---|---|
| Journal | Archives of Oral Biology |
| Volume | 35 |
| Issue number | SUPPL. |
| DOIs | |
| State | Published - 1990 |
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Keywords
- ammonia
- degranulation
- neutrophils
- oxygen metabolism
- periodontal disease
- phagocytosis
- polymorphonuclear leucocytes
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Ammonia as a potential mediator of adult human periodontal infection : Inhibition of neutrophil function. / Niederman, Richard; Brunkhorst, B.; Smith, S.; Weinreb, R. N.; Ryder, M. I.
In: Archives of Oral Biology, Vol. 35, No. SUPPL., 1990.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ammonia as a potential mediator of adult human periodontal infection
T2 - Inhibition of neutrophil function
AU - Niederman, Richard
AU - Brunkhorst, B.
AU - Smith, S.
AU - Weinreb, R. N.
AU - Ryder, M. I.
PY - 1990
Y1 - 1990
N2 - Neutrophils (polymorphonuclear leucocytes) are the principal cell of the host defence system. Consequently, if periodontal pathogen-derived substances in the gingival crevice significantly inhibit their function, they could shift the bacterial-host balance in favour of the bacteria. The hypothesis that ammonia can inhibit neutrophil function was tested. Ammonia was specifically selected because periodontal pathogens produce substantial amounts of ammonia. The findings indicated that ammonia can inhibit neutrophil phagocytosis, degranulation and oxygen metabolism. Ammonia decreased the total number of phagocytosing polymorphonuclear neutrophils (66% of control) and also decreased degranulation (61% of control). Ammonia decreased oxygen metabolism of both resting and stimulated neutrophils (33 and 42% of control, respectively). These observations support the hypothesis that ammonia can inhibit the function of polymorphonuclear leucocytes. They suggest that the presence of ammonia in the gingival crevice may increase the risk of development of periodonal disease.
AB - Neutrophils (polymorphonuclear leucocytes) are the principal cell of the host defence system. Consequently, if periodontal pathogen-derived substances in the gingival crevice significantly inhibit their function, they could shift the bacterial-host balance in favour of the bacteria. The hypothesis that ammonia can inhibit neutrophil function was tested. Ammonia was specifically selected because periodontal pathogens produce substantial amounts of ammonia. The findings indicated that ammonia can inhibit neutrophil phagocytosis, degranulation and oxygen metabolism. Ammonia decreased the total number of phagocytosing polymorphonuclear neutrophils (66% of control) and also decreased degranulation (61% of control). Ammonia decreased oxygen metabolism of both resting and stimulated neutrophils (33 and 42% of control, respectively). These observations support the hypothesis that ammonia can inhibit the function of polymorphonuclear leucocytes. They suggest that the presence of ammonia in the gingival crevice may increase the risk of development of periodonal disease.
KW - ammonia
KW - degranulation
KW - neutrophils
KW - oxygen metabolism
KW - periodontal disease
KW - phagocytosis
KW - polymorphonuclear leucocytes
UR - http://www.scopus.com/inward/record.url?scp=0025664007&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025664007&partnerID=8YFLogxK
U2 - 10.1016/0003-9969(90)90159-8
DO - 10.1016/0003-9969(90)90159-8
M3 - Article
C2 - 2088228
AN - SCOPUS:0025664007
VL - 35
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - SUPPL.
ER -