Alternative splicing induced by insertion of retrotransposons into the maize waxy gene

Marguerite J. Varagona, Michael Purugganan, Susan R. Wessler

Research output: Contribution to journalArticle

Abstract

The molecular basis for the low level expression of three alleles of the maize waxy (Wx) gene has been described. Each allele contains a retrotransposon in intron sequences. These insertions represent previously undescribed elements their association with three wx alleles indicates that retrotransposon elements are important agents of spontaneous mutation in maize. For each allele, element sequences are spliced from pre-mRNA with the surrounding intron even though the insertions increase intron length by pproximately 40- to 60-fold. In addition, despite deferences in element sequences insertion sites, and relative orientations, each element disrupts long-range splice site recogniton leading to novel Wx transcripts where exons both upstream and downstream of the insertion site are skipped. The expression of wx alleles with large insertions in introns provides support for studies that indicate that the primary cis requirement for maize introns is the splice donor and acceptor sites.

Original languageEnglish (US)
Pages (from-to)811-820
Number of pages10
JournalPlant Cell
Volume4
Issue number7
StatePublished - Jul 1992

Fingerprint

waxy corn
Retroelements
alternative splicing
retrotransposons
Alternative Splicing
Introns
Zea mays
introns
Genes
Alleles
alleles
RNA Splice Sites
genes
DNA Transposable Elements
corn
RNA Precursors
Chemical elements
transposons
exons
Exons

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Alternative splicing induced by insertion of retrotransposons into the maize waxy gene. / Varagona, Marguerite J.; Purugganan, Michael; Wessler, Susan R.

In: Plant Cell, Vol. 4, No. 7, 07.1992, p. 811-820.

Research output: Contribution to journalArticle

Varagona, Marguerite J. ; Purugganan, Michael ; Wessler, Susan R. / Alternative splicing induced by insertion of retrotransposons into the maize waxy gene. In: Plant Cell. 1992 ; Vol. 4, No. 7. pp. 811-820.
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