Altered expression of tropomodulin in cardiomyocytes disrupts the sarcomeric structure of myofibrils

Mark A. Sussman, Susanna Baqué, Chang Sub Uhm, Mathew P. Daniels, Robert L. Price, David Simpson, Louis Terracio, Larry Kedes

Research output: Contribution to journalArticle

Abstract

Tropomodulin is a tropomyosin-binding protein that terminates 'pointed- end' actin filament polymerization. To test the hypothesis that regulation of tropomodulin:actin filament stoichiometry is critical for maintenance of actin filament length, tropomodulin levels were altered in cells by infection with recombinant adenoviral expression vectors, which produce either sense or antisense tropomodulin mRNA. Neonatal rat cardiomyocytes were infected, and sarcomeric actin filament organization was examined. Confocal microscopy indicated that overexpression of tropomodulin protein shortened actin filaments and caused myofibril degeneration. In contrast, decreased tropomodulin content resulted in the formation of abnormally long actin filament bundles. Despite changes in myofibril structure caused by altered tropomodulin expression, total protein turnover of the cardiomyocytes was unaffected. Biochemical analyses of infected cardiomyocytes indicated that changes in actin distribution, rather than altered actin content, accounted for myofibril reorganization. Ultrastructural analysis showed thin-filament disarray and revealed the presence of leptomeres after tropomodulin overexpression. Tropomodulin-mediated effects constitute a novel mechanism to control actin filaments, and our findings demonstrate that regulated tropomodulin expression is necessary to maintain stabilized actin filament structures in cardiac muscle cells.

Original languageEnglish (US)
Pages (from-to)94-105
Number of pages12
JournalCirculation research
Volume82
Issue number1
DOIs
StatePublished - Jan 1 1998

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Keywords

  • Actin
  • Cardiac, heart
  • Myofibril
  • Sarcomere
  • Tropomodulin

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Sussman, M. A., Baqué, S., Uhm, C. S., Daniels, M. P., Price, R. L., Simpson, D., Terracio, L., & Kedes, L. (1998). Altered expression of tropomodulin in cardiomyocytes disrupts the sarcomeric structure of myofibrils. Circulation research, 82(1), 94-105. https://doi.org/10.1161/01.RES.82.1.94