Altered cyclic adenosine 3': 5'-monophosphate metabolism and enzymatic profiles of a transplantable murine submaxillary gland carcinoma (myoepithelioma)

Daniel Malamud, D. Irwin

Research output: Contribution to journalArticle

Abstract

The cyclic nucleotide system has been characterized in a transplantable murine submaxillary gland carcinoma. Thymidine incorporation into tumour cell DNA during log phase growth was 10-fold greater than in normal submaxillary gland and was not affected by isoproterenol (IPR). Injection of IPR increased thymidine incorporation into submaxillary gland DNA 6-fold. Tumour adenylate cyclase activity was depressed relative to submaxillary gland and was not stimulated by IPR. Submaxillary gland adenylate cyclase activity was increased 3-fold in response to 10-4 MIPR. The tumour high-K(m) cytosol adenosine 3':5'-phosphate and cyclic guanosine 3':5'-phosphate phosphodiesterase activities were elevated 2- and 9-fold, respectively, over submaxillary gland. Consequently, endogenous cyclic adenosine 3':5'-phosphate levels in the tumour were about one-third the level found in normal salivary glands. Normal submaxillary gland secretory products (amylase and epidermal and nerve growth factors) were all reduced in tumour preparations. The carcinoma can thus be characterized as a rapidly dividing, malignant tumour with decreased levels of submaxillary gland secretory products and a loss in the ability to respond to β-adrenergic stimulation.

Original languageEnglish (US)
Pages (from-to)2945-2949
Number of pages5
JournalCancer Research
Volume38
Issue number9
StatePublished - 1978

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Myoepithelioma
Submandibular Gland
Adenosine
Carcinoma
Adenosine Monophosphate
Isoproterenol
Neoplasms
Adenylyl Cyclases
Thymidine
Type 5 Cyclic Nucleotide Phosphodiesterases
Cyclic Nucleotides
DNA
Nerve Growth Factors
Amylases
Salivary Glands
Adrenergic Agents
Cytosol

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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title = "Altered cyclic adenosine 3': 5'-monophosphate metabolism and enzymatic profiles of a transplantable murine submaxillary gland carcinoma (myoepithelioma)",
abstract = "The cyclic nucleotide system has been characterized in a transplantable murine submaxillary gland carcinoma. Thymidine incorporation into tumour cell DNA during log phase growth was 10-fold greater than in normal submaxillary gland and was not affected by isoproterenol (IPR). Injection of IPR increased thymidine incorporation into submaxillary gland DNA 6-fold. Tumour adenylate cyclase activity was depressed relative to submaxillary gland and was not stimulated by IPR. Submaxillary gland adenylate cyclase activity was increased 3-fold in response to 10-4 MIPR. The tumour high-K(m) cytosol adenosine 3':5'-phosphate and cyclic guanosine 3':5'-phosphate phosphodiesterase activities were elevated 2- and 9-fold, respectively, over submaxillary gland. Consequently, endogenous cyclic adenosine 3':5'-phosphate levels in the tumour were about one-third the level found in normal salivary glands. Normal submaxillary gland secretory products (amylase and epidermal and nerve growth factors) were all reduced in tumour preparations. The carcinoma can thus be characterized as a rapidly dividing, malignant tumour with decreased levels of submaxillary gland secretory products and a loss in the ability to respond to β-adrenergic stimulation.",
author = "Daniel Malamud and D. Irwin",
year = "1978",
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T2 - 5'-monophosphate metabolism and enzymatic profiles of a transplantable murine submaxillary gland carcinoma (myoepithelioma)

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AU - Irwin, D.

PY - 1978

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N2 - The cyclic nucleotide system has been characterized in a transplantable murine submaxillary gland carcinoma. Thymidine incorporation into tumour cell DNA during log phase growth was 10-fold greater than in normal submaxillary gland and was not affected by isoproterenol (IPR). Injection of IPR increased thymidine incorporation into submaxillary gland DNA 6-fold. Tumour adenylate cyclase activity was depressed relative to submaxillary gland and was not stimulated by IPR. Submaxillary gland adenylate cyclase activity was increased 3-fold in response to 10-4 MIPR. The tumour high-K(m) cytosol adenosine 3':5'-phosphate and cyclic guanosine 3':5'-phosphate phosphodiesterase activities were elevated 2- and 9-fold, respectively, over submaxillary gland. Consequently, endogenous cyclic adenosine 3':5'-phosphate levels in the tumour were about one-third the level found in normal salivary glands. Normal submaxillary gland secretory products (amylase and epidermal and nerve growth factors) were all reduced in tumour preparations. The carcinoma can thus be characterized as a rapidly dividing, malignant tumour with decreased levels of submaxillary gland secretory products and a loss in the ability to respond to β-adrenergic stimulation.

AB - The cyclic nucleotide system has been characterized in a transplantable murine submaxillary gland carcinoma. Thymidine incorporation into tumour cell DNA during log phase growth was 10-fold greater than in normal submaxillary gland and was not affected by isoproterenol (IPR). Injection of IPR increased thymidine incorporation into submaxillary gland DNA 6-fold. Tumour adenylate cyclase activity was depressed relative to submaxillary gland and was not stimulated by IPR. Submaxillary gland adenylate cyclase activity was increased 3-fold in response to 10-4 MIPR. The tumour high-K(m) cytosol adenosine 3':5'-phosphate and cyclic guanosine 3':5'-phosphate phosphodiesterase activities were elevated 2- and 9-fold, respectively, over submaxillary gland. Consequently, endogenous cyclic adenosine 3':5'-phosphate levels in the tumour were about one-third the level found in normal salivary glands. Normal submaxillary gland secretory products (amylase and epidermal and nerve growth factors) were all reduced in tumour preparations. The carcinoma can thus be characterized as a rapidly dividing, malignant tumour with decreased levels of submaxillary gland secretory products and a loss in the ability to respond to β-adrenergic stimulation.

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