All optical interface for parallel, remote, and spatiotemporal control of neuronal activity

Sheng Wang, Stephanie Szobota, Yuan Wang, Matthew Volgraf, Zhaowei Liu, Cheng Sun, Dirk Trauner, Ehud Y. Isacoff, Xiang Zhang

Research output: Contribution to journalArticle

Abstract

A key technical barrier to furthering our understanding of complex neural networks has been the lack of tools for the simultaneous spatiotemporal control and detection of activity in a large number of-neurons. Here, we report an all-optical system for achieving this kind of parallel and selective control and detection. We do this by delivering spatiotemporally complex optical stimuli through a digital micromirror spatiotemporal light modulator to cells expressing the light-activated ionotropic glutamate receptor (LiGluR), which have been labeled with a calcium dye to provide a fluorescent report of activity. Reliable and accurate spatiotemporal stimulation was obtained on HEK293 cells and cultured rat hippocampal neurons. This technique should be adaptable to in vivo applications and could serve as an optical interface for communicating with complex neural circuits.

Original languageEnglish (US)
Pages (from-to)3859-3863
Number of pages5
JournalNano Letters
Volume7
Issue number12
DOIs
StatePublished - Dec 2007

Fingerprint

remote control
neurons
Neurons
Ionotropic Glutamate Receptors
communicating
glutamates
Light modulators
light modulators
stimulation
Optical systems
stimuli
rats
Rats
calcium
Calcium
Coloring Agents
Dyes
dyes
Cells
Neural networks

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Wang, S., Szobota, S., Wang, Y., Volgraf, M., Liu, Z., Sun, C., ... Zhang, X. (2007). All optical interface for parallel, remote, and spatiotemporal control of neuronal activity. Nano Letters, 7(12), 3859-3863. https://doi.org/10.1021/nl072783t

All optical interface for parallel, remote, and spatiotemporal control of neuronal activity. / Wang, Sheng; Szobota, Stephanie; Wang, Yuan; Volgraf, Matthew; Liu, Zhaowei; Sun, Cheng; Trauner, Dirk; Isacoff, Ehud Y.; Zhang, Xiang.

In: Nano Letters, Vol. 7, No. 12, 12.2007, p. 3859-3863.

Research output: Contribution to journalArticle

Wang, S, Szobota, S, Wang, Y, Volgraf, M, Liu, Z, Sun, C, Trauner, D, Isacoff, EY & Zhang, X 2007, 'All optical interface for parallel, remote, and spatiotemporal control of neuronal activity', Nano Letters, vol. 7, no. 12, pp. 3859-3863. https://doi.org/10.1021/nl072783t
Wang, Sheng ; Szobota, Stephanie ; Wang, Yuan ; Volgraf, Matthew ; Liu, Zhaowei ; Sun, Cheng ; Trauner, Dirk ; Isacoff, Ehud Y. ; Zhang, Xiang. / All optical interface for parallel, remote, and spatiotemporal control of neuronal activity. In: Nano Letters. 2007 ; Vol. 7, No. 12. pp. 3859-3863.
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