Afterglow solid-state NMR spectroscopy

Gili Abramov, Nathaniel Traaseth

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Biomolecular solid-state NMR experiments have traditionally been collected through detection of 13C or 15N nuclei. Since these nuclei have relatively low sensitivity stemming from their smaller gyromagnetic ratios relative to 1H, the time required to collect multi-dimensional datasets serves as a limitation to resonance assignment and structure determination. One improvement in the field has been to employ simultaneous or parallel acquisition techniques with the goal of acquiring more than one dataset at a time and therefore speeding up the overall data collection process. Central to these experiments is the cross-polarization (CP) element, which serves as a way to transfer magnetization between nuclei via magnetic dipolar couplings. In this chapter, we show how residual signal remaining after CP is a polarization source that can be used to acquire additional datasets. The setup of this class of experiments, referred to as Afterglow spectroscopy, is described and demonstrated using a membrane protein transporter involved in multidrug resistance.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages55-66
Number of pages12
Volume1688
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1688
ISSN (Print)1064-3745

Fingerprint

Magnetic Resonance Spectroscopy
Membrane Transport Proteins
Multiple Drug Resistance
Spectrum Analysis
Membrane Proteins
Datasets

Keywords

  • Magic-angle-spinning
  • Membrane proteins
  • Multidrug resistance
  • Multiple receiver detection
  • NMR spectroscopy
  • Sensitivity enhancement
  • Solid-state NMR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Abramov, G., & Traaseth, N. (2018). Afterglow solid-state NMR spectroscopy. In Methods in Molecular Biology (Vol. 1688, pp. 55-66). (Methods in Molecular Biology; Vol. 1688). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7386-6_3

Afterglow solid-state NMR spectroscopy. / Abramov, Gili; Traaseth, Nathaniel.

Methods in Molecular Biology. Vol. 1688 Humana Press Inc., 2018. p. 55-66 (Methods in Molecular Biology; Vol. 1688).

Research output: Chapter in Book/Report/Conference proceedingChapter

Abramov, G & Traaseth, N 2018, Afterglow solid-state NMR spectroscopy. in Methods in Molecular Biology. vol. 1688, Methods in Molecular Biology, vol. 1688, Humana Press Inc., pp. 55-66. https://doi.org/10.1007/978-1-4939-7386-6_3
Abramov G, Traaseth N. Afterglow solid-state NMR spectroscopy. In Methods in Molecular Biology. Vol. 1688. Humana Press Inc. 2018. p. 55-66. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7386-6_3
Abramov, Gili ; Traaseth, Nathaniel. / Afterglow solid-state NMR spectroscopy. Methods in Molecular Biology. Vol. 1688 Humana Press Inc., 2018. pp. 55-66 (Methods in Molecular Biology).
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