Adhesion-Dependent Wave Generation in Crawling Cells

Erin L. Barnhart, Jun Allard, Sunny S. Lou, Julie A. Theriot, Alexander Mogilner

Research output: Contribution to journalArticle

Abstract

Dynamic actin networks are excitable. In migrating cells, feedback loops can amplify stochastic fluctuations in actin dynamics, often resulting in traveling waves of protrusion. The precise contributions of various molecular and mechanical interactions to wave generation have been difficult to disentangle, in part due to complex cellular morphodynamics. Here we used a relatively simple cell type—the fish epithelial keratocyte—to define a set of mechanochemical feedback loops underlying actin network excitability and wave generation. Although keratocytes are normally characterized by the persistent protrusion of a broad leading edge, increasing cell-substrate adhesion strength results in waving protrusion of a short leading edge. We show that protrusion waves are due to fluctuations in actin polymerization rates and that overexpression of VASP, an actin anti-capping protein that promotes actin polymerization, switches highly adherent keratocytes from waving to persistent protrusion. Moreover, VASP localizes both to adhesion complexes and to the leading edge. Based on these results, we developed a mathematical model for protrusion waves in which local depletion of VASP from the leading edge by adhesions—along with lateral propagation of protrusion due to the branched architecture of the actin network and negative mechanical feedback from the cell membrane—results in regular protrusion waves. Consistent with our model simulations, we show that VASP localization at the leading edge oscillates, with VASP leading-edge enrichment greatest just prior to protrusion initiation. We propose that the mechanochemical feedbacks underlying wave generation in keratocytes may constitute a general module for establishing excitable actin dynamics in other cellular contexts.

Original languageEnglish (US)
Pages (from-to)27-38
Number of pages12
JournalCurrent Biology
Volume27
Issue number1
DOIs
StatePublished - Jan 9 2017

Fingerprint

actin
adhesion
Actins
Adhesion
Feedback
cells
Polymerization
polymerization
Actin Capping Proteins
Bond strength (materials)
Cell Adhesion
Fish
Fishes
Theoretical Models
Switches
simulation models
Mathematical models
mathematical models
Substrates
fish

Keywords

  • actin dynamics
  • actin waves
  • adhesion dynamics
  • cell motility
  • excitable system
  • keratocyte
  • leading edge
  • VASP

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Adhesion-Dependent Wave Generation in Crawling Cells. / Barnhart, Erin L.; Allard, Jun; Lou, Sunny S.; Theriot, Julie A.; Mogilner, Alexander.

In: Current Biology, Vol. 27, No. 1, 09.01.2017, p. 27-38.

Research output: Contribution to journalArticle

Barnhart, EL, Allard, J, Lou, SS, Theriot, JA & Mogilner, A 2017, 'Adhesion-Dependent Wave Generation in Crawling Cells', Current Biology, vol. 27, no. 1, pp. 27-38. https://doi.org/10.1016/j.cub.2016.11.011
Barnhart, Erin L. ; Allard, Jun ; Lou, Sunny S. ; Theriot, Julie A. ; Mogilner, Alexander. / Adhesion-Dependent Wave Generation in Crawling Cells. In: Current Biology. 2017 ; Vol. 27, No. 1. pp. 27-38.
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