Actomyosin-dependent cortical dynamics contributes to the prophase force-balance in the Early Drosophila embryo

Patrizia Sommi, Dhanya Cheerambathur, Ingrid Brust-Mascher, Alex Mogilner

Research output: Contribution to journalArticle

Abstract

Background: The assembly of the Drosophila embryo mitotic spindle during prophase depends upon a balance of outward forces generated by cortical dynein and inward forces generated by kinesin-14 and nuclear elasticity. Myosin II is known to contribute to the dynamics of the cell cortex but how this influences the prophase force-balance is unclear. Principal Findings: Here we investigated this question by injecting the myosin II inhibitor, Y27632, into early Drosophila embryos. We observed a significant increase in both the area of the dense cortical actin caps and in the spacing of the spindle poles. Tracking of microtubule plus ends marked by EB1-GFP and of actin at the cortex revealed that astral microtubules can interact with all regions of these expanded caps, presumably via their interaction with cortical dynein. In Scrambled mutants displaying abnormally small actin caps but normal prophase spindle length in late prophase, myosin II inhibition produced very short spindles. Conclusions: These results suggest that two complementary outward forces are exerted on the prophase spindle by the overlying cortex. Specifically, dynein localized on the mechanically firm actin caps and the actomyosin-driven contraction of the deformable soft patches of the actin cortex, cooperate to pull astral microtubules outward. Thus, myosin II controls the size and dynamic properties of the actin-based cortex to influence the spacing of the poles of the underlying spindle during prophase.

Original languageEnglish (US)
Article numbere18366
JournalPLoS One
Volume6
Issue number3
DOIs
StatePublished - 2011

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Prophase
Actomyosin
prophase
Myosin Type II
Drosophila
actin
Actins
embryo (animal)
Embryonic Structures
myosin
cortex
Dyneins
Microtubules
Spindle Poles
microtubules
Poles
spatial distribution
Kinesin
kinesin
mitotic spindle apparatus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Actomyosin-dependent cortical dynamics contributes to the prophase force-balance in the Early Drosophila embryo. / Sommi, Patrizia; Cheerambathur, Dhanya; Brust-Mascher, Ingrid; Mogilner, Alex.

In: PLoS One, Vol. 6, No. 3, e18366, 2011.

Research output: Contribution to journalArticle

Sommi, Patrizia ; Cheerambathur, Dhanya ; Brust-Mascher, Ingrid ; Mogilner, Alex. / Actomyosin-dependent cortical dynamics contributes to the prophase force-balance in the Early Drosophila embryo. In: PLoS One. 2011 ; Vol. 6, No. 3.
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