Activity of the mitochondrial multiple conductance channel is independent of the adenine nucleotide translocator

Timothy A. Lohret, Robert C. Murphy, Tomas Drgoñ, Kathleen W. Kinnally

Research output: Contribution to journalArticle

Abstract

The functional relationship between the adenine nucleotide translocator (ANT) and the mitochondrial multiple conductance channel (MCC) was investigated using patch-clamp techniques. MCC activity with the same conductance, ion selectivity, voltage dependence, and peptide sensitivity could be reconstituted from inner membrane fractions derived from mitochondria of ANT-deficient and wild-type Saccharomyces cerevisiae. In addition, the MCC activity of mouse kidney mitoplasts was unaffected by carboxyatractyloside, a known inhibitor of ANT and inducer of a permeability transition. These results suggest that MCC activity is independent of ANT.

Original languageEnglish (US)
Pages (from-to)4846-4849
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number9
DOIs
StatePublished - Mar 1 1996

Fingerprint

Adenine Nucleotides
Mitochondria
Clamping devices
Patch-Clamp Techniques
Yeast
Saccharomyces cerevisiae
Permeability
Ions
Membranes
Kidney
Peptides
Electric potential

ASJC Scopus subject areas

  • Biochemistry

Cite this

Activity of the mitochondrial multiple conductance channel is independent of the adenine nucleotide translocator. / Lohret, Timothy A.; Murphy, Robert C.; Drgoñ, Tomas; Kinnally, Kathleen W.

In: Journal of Biological Chemistry, Vol. 271, No. 9, 01.03.1996, p. 4846-4849.

Research output: Contribution to journalArticle

Lohret, Timothy A. ; Murphy, Robert C. ; Drgoñ, Tomas ; Kinnally, Kathleen W. / Activity of the mitochondrial multiple conductance channel is independent of the adenine nucleotide translocator. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 9. pp. 4846-4849.
@article{0c417edbfde343de94c0efef4d6bdbef,
title = "Activity of the mitochondrial multiple conductance channel is independent of the adenine nucleotide translocator",
abstract = "The functional relationship between the adenine nucleotide translocator (ANT) and the mitochondrial multiple conductance channel (MCC) was investigated using patch-clamp techniques. MCC activity with the same conductance, ion selectivity, voltage dependence, and peptide sensitivity could be reconstituted from inner membrane fractions derived from mitochondria of ANT-deficient and wild-type Saccharomyces cerevisiae. In addition, the MCC activity of mouse kidney mitoplasts was unaffected by carboxyatractyloside, a known inhibitor of ANT and inducer of a permeability transition. These results suggest that MCC activity is independent of ANT.",
author = "Lohret, {Timothy A.} and Murphy, {Robert C.} and Tomas Drgo{\~n} and Kinnally, {Kathleen W.}",
year = "1996",
month = "3",
day = "1",
doi = "10.1074/jbc.271.9.4846",
language = "English (US)",
volume = "271",
pages = "4846--4849",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "9",

}

TY - JOUR

T1 - Activity of the mitochondrial multiple conductance channel is independent of the adenine nucleotide translocator

AU - Lohret, Timothy A.

AU - Murphy, Robert C.

AU - Drgoñ, Tomas

AU - Kinnally, Kathleen W.

PY - 1996/3/1

Y1 - 1996/3/1

N2 - The functional relationship between the adenine nucleotide translocator (ANT) and the mitochondrial multiple conductance channel (MCC) was investigated using patch-clamp techniques. MCC activity with the same conductance, ion selectivity, voltage dependence, and peptide sensitivity could be reconstituted from inner membrane fractions derived from mitochondria of ANT-deficient and wild-type Saccharomyces cerevisiae. In addition, the MCC activity of mouse kidney mitoplasts was unaffected by carboxyatractyloside, a known inhibitor of ANT and inducer of a permeability transition. These results suggest that MCC activity is independent of ANT.

AB - The functional relationship between the adenine nucleotide translocator (ANT) and the mitochondrial multiple conductance channel (MCC) was investigated using patch-clamp techniques. MCC activity with the same conductance, ion selectivity, voltage dependence, and peptide sensitivity could be reconstituted from inner membrane fractions derived from mitochondria of ANT-deficient and wild-type Saccharomyces cerevisiae. In addition, the MCC activity of mouse kidney mitoplasts was unaffected by carboxyatractyloside, a known inhibitor of ANT and inducer of a permeability transition. These results suggest that MCC activity is independent of ANT.

UR - http://www.scopus.com/inward/record.url?scp=0029981386&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029981386&partnerID=8YFLogxK

U2 - 10.1074/jbc.271.9.4846

DO - 10.1074/jbc.271.9.4846

M3 - Article

VL - 271

SP - 4846

EP - 4849

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 9

ER -