Activity of hippocampal extract on development of [3H]5-HT high-affinity uptake in dissociated microcultures

E. C. Azmitia, P. Lama, M. Segal, P. M. Whitaker-Azmitia, R. B. Murphy, F. C. Zhou

Research output: Contribution to journalArticle

Abstract

Specific and localized lesions of the 5-HT fibers in the hippocampus induce homotypic collateral sprouting and enhance serotonergic fiber outgrowth from adult neurons and transplanted fetal tissue. In this study, hippocampal extracts were prepared and applied to primary cultures of fetal serotonergic neurons. The effects of plating density and serum additives were examined. The growth of the serotonergic neurons in the rostral brainstem dissociated cultures were estimated by measuring the specific uptake of [3H]5-HT. The results indicate the presence of a trypsin-sensitive factor which is active when prepared fresh at dilutions up to 1 10,000. The factor is higher in hippocampus than cerebellum. Young male tissue contained more activity than either female or aged hippocampus. Although both positive and negative effects are described, higher dilutions of factor ( 1 1,000) were generally stimulatory in high density cultures while lower dilutions ( 1 10) were inhibitory in low density cultures. Specific removal of 5-HT hippocampal afferents with fornix-fimbria microinjections of 5,7-dihydroxytryptamine resulted in an initial loss of activity (2 days and 2 weeks) followed by an enhanced activity (2 months) compared to normal hippocampal extract. Several possibilities are discussed as to the identity of the serotonergic growth factor from hippocampal supernatant.

Original languageEnglish (US)
Pages (from-to)251-258
Number of pages8
JournalInternational Journal of Developmental Neuroscience
Volume9
Issue number3
DOIs
StatePublished - 1991

Fingerprint

Serotonergic Neurons
Hippocampus
Serotonin
Brain Fornix
5,7-Dihydroxytryptamine
Microinjections
Trypsin
Cerebellum
Brain Stem
Intercellular Signaling Peptides and Proteins
Fetus
Neurons
Growth
Serum

Keywords

  • 5
  • 7-dihydroxytryptamine
  • aging
  • cell culture
  • extract
  • hippocampus
  • serotonin

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Activity of hippocampal extract on development of [3H]5-HT high-affinity uptake in dissociated microcultures. / Azmitia, E. C.; Lama, P.; Segal, M.; Whitaker-Azmitia, P. M.; Murphy, R. B.; Zhou, F. C.

In: International Journal of Developmental Neuroscience, Vol. 9, No. 3, 1991, p. 251-258.

Research output: Contribution to journalArticle

Azmitia, E. C. ; Lama, P. ; Segal, M. ; Whitaker-Azmitia, P. M. ; Murphy, R. B. ; Zhou, F. C. / Activity of hippocampal extract on development of [3H]5-HT high-affinity uptake in dissociated microcultures. In: International Journal of Developmental Neuroscience. 1991 ; Vol. 9, No. 3. pp. 251-258.
@article{ced5b7b4b05e41e88e03cd17a12daacb,
title = "Activity of hippocampal extract on development of [3H]5-HT high-affinity uptake in dissociated microcultures",
abstract = "Specific and localized lesions of the 5-HT fibers in the hippocampus induce homotypic collateral sprouting and enhance serotonergic fiber outgrowth from adult neurons and transplanted fetal tissue. In this study, hippocampal extracts were prepared and applied to primary cultures of fetal serotonergic neurons. The effects of plating density and serum additives were examined. The growth of the serotonergic neurons in the rostral brainstem dissociated cultures were estimated by measuring the specific uptake of [3H]5-HT. The results indicate the presence of a trypsin-sensitive factor which is active when prepared fresh at dilutions up to 1 10,000. The factor is higher in hippocampus than cerebellum. Young male tissue contained more activity than either female or aged hippocampus. Although both positive and negative effects are described, higher dilutions of factor ( 1 1,000) were generally stimulatory in high density cultures while lower dilutions ( 1 10) were inhibitory in low density cultures. Specific removal of 5-HT hippocampal afferents with fornix-fimbria microinjections of 5,7-dihydroxytryptamine resulted in an initial loss of activity (2 days and 2 weeks) followed by an enhanced activity (2 months) compared to normal hippocampal extract. Several possibilities are discussed as to the identity of the serotonergic growth factor from hippocampal supernatant.",
keywords = "5, 7-dihydroxytryptamine, aging, cell culture, extract, hippocampus, serotonin",
author = "Azmitia, {E. C.} and P. Lama and M. Segal and Whitaker-Azmitia, {P. M.} and Murphy, {R. B.} and Zhou, {F. C.}",
year = "1991",
doi = "10.1016/0736-5748(91)90045-N",
language = "English (US)",
volume = "9",
pages = "251--258",
journal = "International Journal of Developmental Neuroscience",
issn = "0736-5748",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Activity of hippocampal extract on development of [3H]5-HT high-affinity uptake in dissociated microcultures

AU - Azmitia, E. C.

AU - Lama, P.

AU - Segal, M.

AU - Whitaker-Azmitia, P. M.

AU - Murphy, R. B.

AU - Zhou, F. C.

PY - 1991

Y1 - 1991

N2 - Specific and localized lesions of the 5-HT fibers in the hippocampus induce homotypic collateral sprouting and enhance serotonergic fiber outgrowth from adult neurons and transplanted fetal tissue. In this study, hippocampal extracts were prepared and applied to primary cultures of fetal serotonergic neurons. The effects of plating density and serum additives were examined. The growth of the serotonergic neurons in the rostral brainstem dissociated cultures were estimated by measuring the specific uptake of [3H]5-HT. The results indicate the presence of a trypsin-sensitive factor which is active when prepared fresh at dilutions up to 1 10,000. The factor is higher in hippocampus than cerebellum. Young male tissue contained more activity than either female or aged hippocampus. Although both positive and negative effects are described, higher dilutions of factor ( 1 1,000) were generally stimulatory in high density cultures while lower dilutions ( 1 10) were inhibitory in low density cultures. Specific removal of 5-HT hippocampal afferents with fornix-fimbria microinjections of 5,7-dihydroxytryptamine resulted in an initial loss of activity (2 days and 2 weeks) followed by an enhanced activity (2 months) compared to normal hippocampal extract. Several possibilities are discussed as to the identity of the serotonergic growth factor from hippocampal supernatant.

AB - Specific and localized lesions of the 5-HT fibers in the hippocampus induce homotypic collateral sprouting and enhance serotonergic fiber outgrowth from adult neurons and transplanted fetal tissue. In this study, hippocampal extracts were prepared and applied to primary cultures of fetal serotonergic neurons. The effects of plating density and serum additives were examined. The growth of the serotonergic neurons in the rostral brainstem dissociated cultures were estimated by measuring the specific uptake of [3H]5-HT. The results indicate the presence of a trypsin-sensitive factor which is active when prepared fresh at dilutions up to 1 10,000. The factor is higher in hippocampus than cerebellum. Young male tissue contained more activity than either female or aged hippocampus. Although both positive and negative effects are described, higher dilutions of factor ( 1 1,000) were generally stimulatory in high density cultures while lower dilutions ( 1 10) were inhibitory in low density cultures. Specific removal of 5-HT hippocampal afferents with fornix-fimbria microinjections of 5,7-dihydroxytryptamine resulted in an initial loss of activity (2 days and 2 weeks) followed by an enhanced activity (2 months) compared to normal hippocampal extract. Several possibilities are discussed as to the identity of the serotonergic growth factor from hippocampal supernatant.

KW - 5

KW - 7-dihydroxytryptamine

KW - aging

KW - cell culture

KW - extract

KW - hippocampus

KW - serotonin

UR - http://www.scopus.com/inward/record.url?scp=0025763639&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025763639&partnerID=8YFLogxK

U2 - 10.1016/0736-5748(91)90045-N

DO - 10.1016/0736-5748(91)90045-N

M3 - Article

C2 - 1927581

AN - SCOPUS:0025763639

VL - 9

SP - 251

EP - 258

JO - International Journal of Developmental Neuroscience

JF - International Journal of Developmental Neuroscience

SN - 0736-5748

IS - 3

ER -