Activity of Candida rugosa lipase immobilized on γ-Fe2O3 magnetic nanoparticles

Ansil Dyal, Katja Loos, Mayumi Noto, Seung W. Chang, Chiara Spagnoli, Kurikka V P M Shafi, Abraham Ulman, Mary Cowman, Richard A. Gross

Research output: Contribution to journalArticle

Abstract

We report the stability and enzymatic activity of Candida rugosa Lipase (E.C.3.1.1.3) immobilized on γ-Fe2O3 magnetic nanoparticles. The immobilization strategies were either reacting the enzyme amine group with a nanoparticle surface acetyl, or amine groups. In the former, the enzyme was attached through a C=N bond, while in the latter it was connected using glutaraldehyde. AFM images show an average particle size of 20 ± 10 nm after deconvolution. The enzymatic activity of the immobilized lipase was determined by following the ester cleavage of p-nitrophenol butyrate. The covalently immobilized enzyme was stabile and reactive over 30 days.

Original languageEnglish (US)
Pages (from-to)1684-1685
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number7
DOIs
StatePublished - Feb 15 2003

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Candida
Lipases
Lipase
Nanoparticles
Amines
Enzymes
Immobilized Enzymes
Butyrates
Glutaral
Deconvolution
Particle Size
Immobilization
Esters
Particle size
4-nitrophenol

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Dyal, A., Loos, K., Noto, M., Chang, S. W., Spagnoli, C., Shafi, K. V. P. M., ... Gross, R. A. (2003). Activity of Candida rugosa lipase immobilized on γ-Fe2O3 magnetic nanoparticles. Journal of the American Chemical Society, 125(7), 1684-1685. https://doi.org/10.1021/ja021223n

Activity of Candida rugosa lipase immobilized on γ-Fe2O3 magnetic nanoparticles. / Dyal, Ansil; Loos, Katja; Noto, Mayumi; Chang, Seung W.; Spagnoli, Chiara; Shafi, Kurikka V P M; Ulman, Abraham; Cowman, Mary; Gross, Richard A.

In: Journal of the American Chemical Society, Vol. 125, No. 7, 15.02.2003, p. 1684-1685.

Research output: Contribution to journalArticle

Dyal, A, Loos, K, Noto, M, Chang, SW, Spagnoli, C, Shafi, KVPM, Ulman, A, Cowman, M & Gross, RA 2003, 'Activity of Candida rugosa lipase immobilized on γ-Fe2O3 magnetic nanoparticles', Journal of the American Chemical Society, vol. 125, no. 7, pp. 1684-1685. https://doi.org/10.1021/ja021223n
Dyal, Ansil ; Loos, Katja ; Noto, Mayumi ; Chang, Seung W. ; Spagnoli, Chiara ; Shafi, Kurikka V P M ; Ulman, Abraham ; Cowman, Mary ; Gross, Richard A. / Activity of Candida rugosa lipase immobilized on γ-Fe2O3 magnetic nanoparticles. In: Journal of the American Chemical Society. 2003 ; Vol. 125, No. 7. pp. 1684-1685.
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