Active vs. reactive threat responding is associated with differential c-Fos expression in specific regions of amygdala and prefrontal cortex

Raquel C R Martinez, Nikita Gupta, Gabriel Lazaro-Munoz, Robert M. Sears, Soojeong Kim, Justin M. Moscarello, Joseph Ledoux, Christopher K. Cain

Research output: Contribution to journalArticle

Abstract

Active avoidance (AA) is an important paradigm for studying mechanisms of aversive instrumental learning, pathological anxiety, and active coping. Unfortunately, AA neurocircuits are poorly understood, partly because behavior is highly variable and reflects a competition between Pavlovian reactions and instrumental actions. Here we exploited the behavioral differences between good and poor avoiders to elucidate the AA neurocircuit. Rats received Sidman AA training and expression of the activity-dependent immediate-early gene c-fos was measured after a shock-free AA test. Six brain regions with known or putative roles in AA were evaluated: amygdala, periaqueductal gray, nucleus accumbens, dorsal striatum, prefrontal cortex (PFC), and hippocampus. Good avoiders showed little Pavlovian freezing and high AA rates at test, the opposite of poor avoiders. Although c-Fos activation was observed throughout the brain, differential activation was found only in subregions of amygdala and PFC. Interestingly, c-Fos correlated with avoidance and freezing in only five of 20 distinct areas evaluated: lateral amygdala, central amygdala, medial amygdala, basal amygdala, and infralimbic PFC. Thus, activity in specific amygdala-PFC circuits likely mediates the competition between instrumental actions and Pavlovian reactions after AA training. Individual differences in AA behavior, long considered a nuisance by researchers, may be the key to elucidating the AA neurocircuit and understanding pathological response profiles.

Original languageEnglish (US)
Pages (from-to)446-452
Number of pages7
JournalLearning and Memory
Volume20
Issue number8
DOIs
StatePublished - Aug 2013

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Amygdala
Prefrontal Cortex
Freezing
Avoidance Learning
Operant Conditioning
Periaqueductal Gray
Immediate-Early Genes
Nucleus Accumbens
Brain
Individuality
Shock
Hippocampus
Teaching
Anxiety
Research Personnel

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience
  • Neuropsychology and Physiological Psychology
  • Medicine(all)

Cite this

Martinez, R. C. R., Gupta, N., Lazaro-Munoz, G., Sears, R. M., Kim, S., Moscarello, J. M., ... Cain, C. K. (2013). Active vs. reactive threat responding is associated with differential c-Fos expression in specific regions of amygdala and prefrontal cortex. Learning and Memory, 20(8), 446-452. https://doi.org/10.1101/lm.031047.113

Active vs. reactive threat responding is associated with differential c-Fos expression in specific regions of amygdala and prefrontal cortex. / Martinez, Raquel C R; Gupta, Nikita; Lazaro-Munoz, Gabriel; Sears, Robert M.; Kim, Soojeong; Moscarello, Justin M.; Ledoux, Joseph; Cain, Christopher K.

In: Learning and Memory, Vol. 20, No. 8, 08.2013, p. 446-452.

Research output: Contribution to journalArticle

Martinez, Raquel C R ; Gupta, Nikita ; Lazaro-Munoz, Gabriel ; Sears, Robert M. ; Kim, Soojeong ; Moscarello, Justin M. ; Ledoux, Joseph ; Cain, Christopher K. / Active vs. reactive threat responding is associated with differential c-Fos expression in specific regions of amygdala and prefrontal cortex. In: Learning and Memory. 2013 ; Vol. 20, No. 8. pp. 446-452.
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