Active mask segmentation for the cell-volume computation and Golgi-body segmentation of hela cell images

Gowri Srinivasa, Matthew Fickus, Manuel N. Gonzalez-Rivero, Sarah Yichia Hsieh, Yusong Guo, Adam D. Linstedt, Jelena Kovacevic

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a novel active mask framework for the segmentation of fluorescence microscope images of cells, and in particular, for the segmentation of the Golgi body as well as cell-volume computation. We demonstrate that the algorithm is able to efficiently segment a stack of images and successfully assign multiple pieces of the Golgi body in a 2D image to the cell to which they belong. Further, we demonstrate that our algorithm is more accurate than manual segmentation of these images.

Original languageEnglish (US)
Title of host publication2008 5th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, Proceedings, ISBI
Pages348-351
Number of pages4
DOIs
StatePublished - Sep 10 2008
Event2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI - Paris, France
Duration: May 14 2008May 17 2008

Other

Other2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI
CountryFrance
CityParis
Period5/14/085/17/08

Fingerprint

Masks
Microscopes
Fluorescence

Keywords

  • Active contours
  • Active mask
  • Segmentation

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Srinivasa, G., Fickus, M., Gonzalez-Rivero, M. N., Hsieh, S. Y., Guo, Y., Linstedt, A. D., & Kovacevic, J. (2008). Active mask segmentation for the cell-volume computation and Golgi-body segmentation of hela cell images. In 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI (pp. 348-351). [4541004] https://doi.org/10.1109/ISBI.2008.4541004

Active mask segmentation for the cell-volume computation and Golgi-body segmentation of hela cell images. / Srinivasa, Gowri; Fickus, Matthew; Gonzalez-Rivero, Manuel N.; Hsieh, Sarah Yichia; Guo, Yusong; Linstedt, Adam D.; Kovacevic, Jelena.

2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI. 2008. p. 348-351 4541004.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Srinivasa, G, Fickus, M, Gonzalez-Rivero, MN, Hsieh, SY, Guo, Y, Linstedt, AD & Kovacevic, J 2008, Active mask segmentation for the cell-volume computation and Golgi-body segmentation of hela cell images. in 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI., 4541004, pp. 348-351, 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI, Paris, France, 5/14/08. https://doi.org/10.1109/ISBI.2008.4541004
Srinivasa G, Fickus M, Gonzalez-Rivero MN, Hsieh SY, Guo Y, Linstedt AD et al. Active mask segmentation for the cell-volume computation and Golgi-body segmentation of hela cell images. In 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI. 2008. p. 348-351. 4541004 https://doi.org/10.1109/ISBI.2008.4541004
Srinivasa, Gowri ; Fickus, Matthew ; Gonzalez-Rivero, Manuel N. ; Hsieh, Sarah Yichia ; Guo, Yusong ; Linstedt, Adam D. ; Kovacevic, Jelena. / Active mask segmentation for the cell-volume computation and Golgi-body segmentation of hela cell images. 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI. 2008. pp. 348-351
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