Active CMOS array sensor for time-resolved fluorescence detection

George Patounakis, Kenneth L. Shepard, Rastislav Levicky

Research output: Contribution to journalArticle

Abstract

Surface-based sensing assays based on fluorescence-based detection have become commonplace for both environmental and biomedical diagnostics. Traditional array scanners are expensive, large, and complex instruments. This paper describes the design of an active CMOS biosensor substrate for fluorescence-based assays that enables time-gated, time-resolved fluorescence spectroscopy without the need for an external reader. The array is sensitive to photon densities as low as 1.15 x 10s/cm3, has a dynamic range of over 74 dB, and has subnanosecond timing resolution. Sensitivity is achieved through subsampling and averaging.

Original languageEnglish (US)
Article number1717675
Pages (from-to)2521-2530
Number of pages10
JournalIEEE Journal of Solid-State Circuits
Volume41
Issue number11
DOIs
StatePublished - Nov 2006

Fingerprint

Sensor arrays
Assays
Fluorescence
Fluorescence spectroscopy
Biosensors
Photons
Substrates

Keywords

  • Biochips
  • Biosensor
  • CMOS imager
  • Fluorescence spectroscopy
  • Microarray

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Active CMOS array sensor for time-resolved fluorescence detection. / Patounakis, George; Shepard, Kenneth L.; Levicky, Rastislav.

In: IEEE Journal of Solid-State Circuits, Vol. 41, No. 11, 1717675, 11.2006, p. 2521-2530.

Research output: Contribution to journalArticle

Patounakis, George ; Shepard, Kenneth L. ; Levicky, Rastislav. / Active CMOS array sensor for time-resolved fluorescence detection. In: IEEE Journal of Solid-State Circuits. 2006 ; Vol. 41, No. 11. pp. 2521-2530.
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