Active and Inactive Enhancers Cooperate to Exert Localized and Long-Range Control of Gene Regulation

Charlotte Proudhon, Valentina Snetkova, Ramya Raviram, Camille Lobry, Sana Badri, Tingting Jiang, Bingtao Hao, Thomas Trimarchi, Yuval Kluger, Iannis Aifantis, Richard Bonneau, Jane A. Skok

Research output: Contribution to journalArticle

Abstract

V(D)J recombination relies on the presence of proximal enhancers that activate the antigen receptor (AgR) loci in a lineage- and stage-specific manner. Unexpectedly, we find that both active and inactive AgR enhancers cooperate to disseminate their effects in a localized and long-range manner. Here, we demonstrate the importance of short-range contacts between active enhancers that constitute an Igk super-enhancer in B cells. Deletion of one element reduces the interaction frequency between other enhancers in the hub, which compromises the transcriptional output of each component. Furthermore, we establish that, in T cells, long-range contact and cooperation between the inactive Igk enhancer MiEκ and the active Tcrb enhancer Eβ alters enrichment of CBFβ binding in a manner that impacts Tcrb recombination. These findings underline the complexities of enhancer regulation and point to a role for localized and long-range enhancer-sharing between active and inactive elements in lineage- and stage-specific control.

Original languageEnglish (US)
Pages (from-to)2159-2169
Number of pages11
JournalCell Reports
Volume15
Issue number10
DOIs
StatePublished - Jun 7 2016

Fingerprint

Antigen Receptors
Gene expression
V(D)J Recombination
T-cells
Genetic Recombination
Genes
B-Lymphocytes
Cells
T-Lymphocytes

Keywords

  • Enhancer-sharing
  • Gene regulation
  • Igk
  • Localized and long-range contacts
  • Nuclear architecture
  • Super-enhancer
  • Tcrb
  • Transcription factor binding
  • Transcriptional output

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Proudhon, C., Snetkova, V., Raviram, R., Lobry, C., Badri, S., Jiang, T., ... Skok, J. A. (2016). Active and Inactive Enhancers Cooperate to Exert Localized and Long-Range Control of Gene Regulation. Cell Reports, 15(10), 2159-2169. https://doi.org/10.1016/j.celrep.2016.04.087

Active and Inactive Enhancers Cooperate to Exert Localized and Long-Range Control of Gene Regulation. / Proudhon, Charlotte; Snetkova, Valentina; Raviram, Ramya; Lobry, Camille; Badri, Sana; Jiang, Tingting; Hao, Bingtao; Trimarchi, Thomas; Kluger, Yuval; Aifantis, Iannis; Bonneau, Richard; Skok, Jane A.

In: Cell Reports, Vol. 15, No. 10, 07.06.2016, p. 2159-2169.

Research output: Contribution to journalArticle

Proudhon, C, Snetkova, V, Raviram, R, Lobry, C, Badri, S, Jiang, T, Hao, B, Trimarchi, T, Kluger, Y, Aifantis, I, Bonneau, R & Skok, JA 2016, 'Active and Inactive Enhancers Cooperate to Exert Localized and Long-Range Control of Gene Regulation', Cell Reports, vol. 15, no. 10, pp. 2159-2169. https://doi.org/10.1016/j.celrep.2016.04.087
Proudhon, Charlotte ; Snetkova, Valentina ; Raviram, Ramya ; Lobry, Camille ; Badri, Sana ; Jiang, Tingting ; Hao, Bingtao ; Trimarchi, Thomas ; Kluger, Yuval ; Aifantis, Iannis ; Bonneau, Richard ; Skok, Jane A. / Active and Inactive Enhancers Cooperate to Exert Localized and Long-Range Control of Gene Regulation. In: Cell Reports. 2016 ; Vol. 15, No. 10. pp. 2159-2169.
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