Activation of NFκB is necessary for IL-1β-induced cyclooxygenase-2 (COX-2) expression in human gingival fibroblasts

Sumi Nakao, Yorimasa Ogata, Emi Shimizu-Sasaki, Muneyoshi Yamazaki, Shunsuke Furuyama, Hiroshi Sugiya

Research output: Contribution to journalArticle

Abstract

The immediate-early cyclooxygenase-2 (COX-2) gene encodes an inducible prostaglandin synthase enzyme which is implicated in inflammatory and proliferative diseases. COX-2 is highly induced during cell activation by various factors, including mitogens, hormones and cytokines. Since pro- inflammatory cytokine IL-1β has been shown to induce prostaglandin E2 (PGE2) release in human gingival fibroblasts (HGF), here we analyzed the effect of IL-1β on the expression of COX-2 and the activation of NFκB in HGF. Northern hybridization analysis revealed that IL-1β (200 pg/ml) increased the expression of COX-2 mRNA in HGF. The effect of IL-1β was abrogated by herbimycin A, a protein tyrosine kinase inhibitor, and enhanced by orthovanadate, a protein tyrosine phosphatase inhibitor. IL-1β-induced PGE2 release was blocked by the tyrosine kinase inhibitor and increased by the tyrosine phosphatase inhibitor. The results of transient transfection assays using chimeric constructs of the human COX-2 promoter (nt-1432~+59) ligated to a luciferase reporter gene indicated that IL-1β stimulated the transcriptional activity~1.5-fold. Gel mobility shift assays with a radiolabelled COX-2-NFκB oligonucleotide (nts-223 to -214) revealed an increase in the binding of nuclear proteins from IL-1β-stimulated HGF. This increase of DNA-protein complex formation induced by IL-1β was blocked by herbimycin A and another tyrosine kinase inhibitor, genistein. These results suggest that NFκB is an important transcription factor for IL-1β-induced COX-2 gene expression, and is involved in inducing COX-2 gene transcription through tyrosine phosphorylation in HGF.

Original languageEnglish (US)
Pages (from-to)113-118
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume209
Issue number1-2
StatePublished - 2000

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Keywords

  • COX-2
  • Gene regulation
  • Human gingival fibroblasts
  • IL-1β
  • NFκB
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Nakao, S., Ogata, Y., Shimizu-Sasaki, E., Yamazaki, M., Furuyama, S., & Sugiya, H. (2000). Activation of NFκB is necessary for IL-1β-induced cyclooxygenase-2 (COX-2) expression in human gingival fibroblasts. Molecular and Cellular Biochemistry, 209(1-2), 113-118.