Actin and hnRNP U cooperate for productive transcription by RNA polymerase II

Alexander Kukalev, Ylva Nord, Carina Palmberg, Tomas Bergman, Piergiorgio Percipalle

    Research output: Contribution to journalArticle

    Abstract

    To determine the role of actin-ribonucleoprotein complexes in transcription, we set out to identify novel actin-binding proteins associated with RNA polymerase II (Pol II). Using affinity chromatography on fractionated HeLa cells, we found that hnRNP U binds actin through a short amino acid sequence in its C-terminal domain. Post-transcriptional gene silencing of hnRNP U and nuclear microinjections of a short peptide encompassing the hnRNP U actin-binding sequence inhibited BrUTP incorporation in vivo. In living cells, we found that both actin and hnRNP U are associated with the phosphorylated C-terminal domain of Pol II, and antibodies to actin and hnRNP U blocked Pol II-mediated transcription. Taken together, our results indicate that a general actin-based mechanism is implicated in the transcription of most Pol II genes. Actin in complex with hnRNP U may carry out its regulatory role during the initial phases of transcription activation.

    Original languageEnglish (US)
    Pages (from-to)238-244
    Number of pages7
    JournalNature Structural and Molecular Biology
    Volume12
    Issue number3
    DOIs
    StatePublished - Dec 1 2005

    Fingerprint

    Heterogeneous-Nuclear Ribonucleoprotein U
    RNA Polymerase II
    Actins
    Microfilament Proteins
    Ribonucleoproteins
    Microinjections
    RNA Interference
    Affinity Chromatography
    HeLa Cells
    Transcriptional Activation
    Amino Acid Sequence

    ASJC Scopus subject areas

    • Molecular Biology
    • Structural Biology

    Cite this

    Actin and hnRNP U cooperate for productive transcription by RNA polymerase II. / Kukalev, Alexander; Nord, Ylva; Palmberg, Carina; Bergman, Tomas; Percipalle, Piergiorgio.

    In: Nature Structural and Molecular Biology, Vol. 12, No. 3, 01.12.2005, p. 238-244.

    Research output: Contribution to journalArticle

    Kukalev, A, Nord, Y, Palmberg, C, Bergman, T & Percipalle, P 2005, 'Actin and hnRNP U cooperate for productive transcription by RNA polymerase II', Nature Structural and Molecular Biology, vol. 12, no. 3, pp. 238-244. https://doi.org/10.1038/nsmb904
    Kukalev, Alexander ; Nord, Ylva ; Palmberg, Carina ; Bergman, Tomas ; Percipalle, Piergiorgio. / Actin and hnRNP U cooperate for productive transcription by RNA polymerase II. In: Nature Structural and Molecular Biology. 2005 ; Vol. 12, No. 3. pp. 238-244.
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