Accelerating the mass transport of DNA biomolecules onto DNA microarray for enhanced detection by electrokinetic concentration in a microfluidic chip

Diogo Martins, Xi Wei, Rastislav Levicky, Yong Ak Song

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Morpholinos (MOs) are synthetic nucleic acids analogues with a non-charged backbone of morpholine rings. To enhance the MO-DNA hybridization assay speed, we propose the integration of a MO microarray with an ion concentration polarization (ICP) based microfluidic concentrator. The ICP concentrator collects target biomolecules from a ∼μL fluidic DNA sample and concentrates them electrokinetically into a ∼nL plug located in the vicinity of the MO probes. ICP preconcentration not only reduces the analyte diffusion length but also increases the binding reaction rate, and as a result, ICPenhanced MO microarrays allow much faster hybridization than standard diffusion-limited MO microarrays.

Original languageEnglish (US)
Title of host publicationMicro/Nanofluidics and Lab-on-a-Chip; Nanofluids; Micro/Nanoscale Interfacial Transport Phenomena; Micro/Nanoscale Boiling and Condensation Heat Transfer; Micro/Nanoscale Thermal Radiation; Micro/Nanoscale Energy Devices and Systems
PublisherAmerican Society of Mechanical Engineers
ISBN (Electronic)9780791849651
DOIs
StatePublished - Jan 1 2016
EventASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2016 - Biopolis, Singapore
Duration: Jan 4 2016Jan 6 2016

Publication series

NameASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2016
Volume1

Other

OtherASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2016
CountrySingapore
CityBiopolis
Period1/4/161/6/16

ASJC Scopus subject areas

  • Fluid Flow and Transfer Processes

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  • Cite this

    Martins, D., Wei, X., Levicky, R., & Song, Y. A. (2016). Accelerating the mass transport of DNA biomolecules onto DNA microarray for enhanced detection by electrokinetic concentration in a microfluidic chip. In Micro/Nanofluidics and Lab-on-a-Chip; Nanofluids; Micro/Nanoscale Interfacial Transport Phenomena; Micro/Nanoscale Boiling and Condensation Heat Transfer; Micro/Nanoscale Thermal Radiation; Micro/Nanoscale Energy Devices and Systems [V001T01A007] (ASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2016; Vol. 1). American Society of Mechanical Engineers. https://doi.org/10.1115/MNHMT2016-6562