Accelerating the mass transport of DNA biomolecules onto DNA microarray for enhanced detection by electrokinetic concentration in a microfluidic chip

Diogo Martins, Xi Wei, Rastislav Levicky, Rafael Song

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Morpholinos (MOs) are synthetic nucleic acids analogues with a non-charged backbone of morpholine rings. To enhance the MO-DNA hybridization assay speed, we propose the integration of a MO microarray with an ion concentration polarization (ICP) based microfluidic concentrator. The ICP concentrator collects target biomolecules from a ∼μL fluidic DNA sample and concentrates them electrokinetically into a ∼nL plug located in the vicinity of the MO probes. ICP preconcentration not only reduces the analyte diffusion length but also increases the binding reaction rate, and as a result, ICPenhanced MO microarrays allow much faster hybridization than standard diffusion-limited MO microarrays.

Original languageEnglish (US)
Title of host publicationMicro/Nanofluidics and Lab-on-a-Chip; Nanofluids; Micro/Nanoscale Interfacial Transport Phenomena; Micro/Nanoscale Boiling and Condensation Heat Transfer; Micro/Nanoscale Thermal Radiation; Micro/Nanoscale Energy Devices and Systems
PublisherAmerican Society of Mechanical Engineers
Volume1
ISBN (Print)9780791849651
DOIs
StatePublished - 2016
EventASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2016 - Biopolis, Singapore
Duration: Jan 4 2016Jan 6 2016

Other

OtherASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2016
CountrySingapore
CityBiopolis
Period1/4/161/6/16

Fingerprint

Morpholinos
Biomolecules
Microarrays
Microfluidics
DNA
Mass transfer
Polarization
Ions
Nucleic acids
Fluidics
Reaction rates
Assays
Nucleic Acids

ASJC Scopus subject areas

  • Fluid Flow and Transfer Processes

Cite this

Martins, D., Wei, X., Levicky, R., & Song, R. (2016). Accelerating the mass transport of DNA biomolecules onto DNA microarray for enhanced detection by electrokinetic concentration in a microfluidic chip. In Micro/Nanofluidics and Lab-on-a-Chip; Nanofluids; Micro/Nanoscale Interfacial Transport Phenomena; Micro/Nanoscale Boiling and Condensation Heat Transfer; Micro/Nanoscale Thermal Radiation; Micro/Nanoscale Energy Devices and Systems (Vol. 1). [V001T01A007] American Society of Mechanical Engineers. https://doi.org/10.1115/MNHMT2016-6562

Accelerating the mass transport of DNA biomolecules onto DNA microarray for enhanced detection by electrokinetic concentration in a microfluidic chip. / Martins, Diogo; Wei, Xi; Levicky, Rastislav; Song, Rafael.

Micro/Nanofluidics and Lab-on-a-Chip; Nanofluids; Micro/Nanoscale Interfacial Transport Phenomena; Micro/Nanoscale Boiling and Condensation Heat Transfer; Micro/Nanoscale Thermal Radiation; Micro/Nanoscale Energy Devices and Systems. Vol. 1 American Society of Mechanical Engineers, 2016. V001T01A007.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Martins, D, Wei, X, Levicky, R & Song, R 2016, Accelerating the mass transport of DNA biomolecules onto DNA microarray for enhanced detection by electrokinetic concentration in a microfluidic chip. in Micro/Nanofluidics and Lab-on-a-Chip; Nanofluids; Micro/Nanoscale Interfacial Transport Phenomena; Micro/Nanoscale Boiling and Condensation Heat Transfer; Micro/Nanoscale Thermal Radiation; Micro/Nanoscale Energy Devices and Systems. vol. 1, V001T01A007, American Society of Mechanical Engineers, ASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2016, Biopolis, Singapore, 1/4/16. https://doi.org/10.1115/MNHMT2016-6562
Martins D, Wei X, Levicky R, Song R. Accelerating the mass transport of DNA biomolecules onto DNA microarray for enhanced detection by electrokinetic concentration in a microfluidic chip. In Micro/Nanofluidics and Lab-on-a-Chip; Nanofluids; Micro/Nanoscale Interfacial Transport Phenomena; Micro/Nanoscale Boiling and Condensation Heat Transfer; Micro/Nanoscale Thermal Radiation; Micro/Nanoscale Energy Devices and Systems. Vol. 1. American Society of Mechanical Engineers. 2016. V001T01A007 https://doi.org/10.1115/MNHMT2016-6562
Martins, Diogo ; Wei, Xi ; Levicky, Rastislav ; Song, Rafael. / Accelerating the mass transport of DNA biomolecules onto DNA microarray for enhanced detection by electrokinetic concentration in a microfluidic chip. Micro/Nanofluidics and Lab-on-a-Chip; Nanofluids; Micro/Nanoscale Interfacial Transport Phenomena; Micro/Nanoscale Boiling and Condensation Heat Transfer; Micro/Nanoscale Thermal Radiation; Micro/Nanoscale Energy Devices and Systems. Vol. 1 American Society of Mechanical Engineers, 2016.
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