Absorption difference spectroscopy of chlorophyll a in ethanol solution

Suse Broyde, S. S. Brody, M. Brody

Research output: Contribution to journalArticle

Abstract

Absorption difference spectra between highly concentrated and dilute solutions of chlorophyll a have been measured in ethanol, maintaining the product of concentration and path length constant. A log-log plot of dimer versus monomer concentration, derived from the data, was linear with a slope of 2.0, which is consistent with the interpretation that dimers are present in this solvent. The absorption spectrum of the dimer in ethanol was calculated, and a value of 4.5 ± 0.8 M -1 was obtained for the equilibrium constant for dimerization.

Original languageEnglish (US)
Pages (from-to)183-187
Number of pages5
JournalBBA - Bioenergetics
Volume153
Issue number1
StatePublished - Jan 15 1968

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Dimers
Spectrum Analysis
Ethanol
Spectroscopy
Dimerization
Equilibrium constants
Absorption spectra
Monomers
chlorophyll a

ASJC Scopus subject areas

  • Biophysics
  • Medicine(all)

Cite this

Absorption difference spectroscopy of chlorophyll a in ethanol solution. / Broyde, Suse; Brody, S. S.; Brody, M.

In: BBA - Bioenergetics, Vol. 153, No. 1, 15.01.1968, p. 183-187.

Research output: Contribution to journalArticle

Broyde, Suse ; Brody, S. S. ; Brody, M. / Absorption difference spectroscopy of chlorophyll a in ethanol solution. In: BBA - Bioenergetics. 1968 ; Vol. 153, No. 1. pp. 183-187.
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