Absolute configurations of DNA lesions determined by comparisons of experimental ECD and ORD spectra with DFT calculations

Shuang Ding, Alexander Kolbanovskiy, Alexander Durandin, Conor Crean, Vladimir Shafirovich, Suse Broyde, Nicholas E. Geacintov

Research output: Contribution to journalArticle

Abstract

The usefulness of modern density functional theory (DFT) methods is considered for establishing the absolute configurations of DNA lesions by comparisons of computed and experimentally measured optical rotatory dispersion (ORD) and electronic circular dichroism (ECD) spectra. Two rigid, structurally different DNA lesions (two spiroiminodihydantoin stereoisomers and four equine estrogen 4-hydoxyequilenin-DNA stereoisomeric adducts) have been investigated. In all cases, the signs and shapes of the computed ORD spectra reproduced the experimentally measured ORD spectra, although the magnitudes of the computed and experimental ORD values do not coincide exactly. The computed ECD spectra also reproduced the shapes of the experimental ECD spectra rather well, but are blue-shifted by 10-20 nm. Since the assignments of the absolute configurations of the DNA lesions studied based on computed and experimental ORD and ECD spectra are fully consistent with one another, the computational DFT method shows significant promise for determining the absolute configurations of DNA lesions. Establishing the stereochemistry of DNA lesions is highly useful for understanding their biological impact, especially when sufficient amounts of material are not available for other methods of structural characterization.

Original languageEnglish (US)
JournalChirality
Volume21
Issue number1 E
DOIs
StatePublished - 2009

Fingerprint

Optical Rotatory Dispersion
Dichroism
Circular Dichroism
Density functional theory
DNA
Stereochemistry
Stereoisomerism
DNA Adducts
Horses
Estrogens

Keywords

  • 4-hydoxyequilenin
  • DFT
  • DNA adducts
  • Electronic circular dichroism
  • Equine estrogen
  • Optical rotatory dispersion
  • Spiroiminodihydantoin
  • Stereochemistry

ASJC Scopus subject areas

  • Organic Chemistry
  • Analytical Chemistry
  • Drug Discovery
  • Pharmacology
  • Catalysis
  • Spectroscopy

Cite this

Absolute configurations of DNA lesions determined by comparisons of experimental ECD and ORD spectra with DFT calculations. / Ding, Shuang; Kolbanovskiy, Alexander; Durandin, Alexander; Crean, Conor; Shafirovich, Vladimir; Broyde, Suse; Geacintov, Nicholas E.

In: Chirality, Vol. 21, No. 1 E, 2009.

Research output: Contribution to journalArticle

Ding, Shuang ; Kolbanovskiy, Alexander ; Durandin, Alexander ; Crean, Conor ; Shafirovich, Vladimir ; Broyde, Suse ; Geacintov, Nicholas E. / Absolute configurations of DNA lesions determined by comparisons of experimental ECD and ORD spectra with DFT calculations. In: Chirality. 2009 ; Vol. 21, No. 1 E.
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