A zebrafish retinal graded photochemical stress model

Joseph W. Eichenbaum, Ayca Cinaroglu, Kenneth D. Eichenbaum, Kirsten Sadler Edepli

    Research output: Contribution to journalArticle

    Abstract

    Introduction: In order to develop a model for investigating the genes that contribute to retinal degeneration, we examined the early graded photochemical stress response in the adult zebrafish (Danio rerio) retina and investigated the role of an NMDA inhibitor, thiokynurenate. Methods: Following intravitreal injection of rose bengal (6 or 12 mg/mL), light (37 × 103 or 83 × 103 lx) was directed onto the central retina with and without 400 nM thiokynurenate. Histologic and electron microscopic analysis was performed at 2 and 4 h and gene expression analysis was carried out at 2, 4 and 6 h. Results: Light and electron microscopy demonstrated a graded photochemical response in photoreceptor, nuclear, and ganglion cell layer thickness. Increased vacuolation of the inner plexiform layer was also observed. The inhibitor produced a distinct lesion pattern. Cellular stress genes were elevated in low and high lesions, while some homeobox gene expression was reduced with thiokynurenate. Discussion: The phenotypic and genetic changes observed from this model can serve as a basis for understanding the pathology of retinal oxidative and cellular stress. These changes may aid our understanding of aging and macular degeneration.

    Original languageEnglish (US)
    Pages (from-to)121-127
    Number of pages7
    JournalJournal of Pharmacological and Toxicological Methods
    Volume59
    Issue number3
    DOIs
    StatePublished - May 1 2009

    Fingerprint

    Zebrafish
    Gene expression
    Retina
    Genes
    Gene Expression
    Rose Bengal
    Light
    Retinal Degeneration
    Intravitreal Injections
    Homeobox Genes
    Macular Degeneration
    Pathology
    N-Methylaspartate
    Ganglia
    Electron microscopy
    Optical microscopy
    Electron Microscopy
    Oxidative Stress
    Aging of materials
    Cells

    Keywords

    • Genes in retinal degeneration
    • NMDA inhibitor
    • Photochemical cellular stress
    • Zebrafish

    ASJC Scopus subject areas

    • Toxicology
    • Pharmacology

    Cite this

    Eichenbaum, J. W., Cinaroglu, A., Eichenbaum, K. D., & Sadler Edepli, K. (2009). A zebrafish retinal graded photochemical stress model. Journal of Pharmacological and Toxicological Methods, 59(3), 121-127. https://doi.org/10.1016/j.vascn.2009.02.006

    A zebrafish retinal graded photochemical stress model. / Eichenbaum, Joseph W.; Cinaroglu, Ayca; Eichenbaum, Kenneth D.; Sadler Edepli, Kirsten.

    In: Journal of Pharmacological and Toxicological Methods, Vol. 59, No. 3, 01.05.2009, p. 121-127.

    Research output: Contribution to journalArticle

    Eichenbaum, JW, Cinaroglu, A, Eichenbaum, KD & Sadler Edepli, K 2009, 'A zebrafish retinal graded photochemical stress model', Journal of Pharmacological and Toxicological Methods, vol. 59, no. 3, pp. 121-127. https://doi.org/10.1016/j.vascn.2009.02.006
    Eichenbaum, Joseph W. ; Cinaroglu, Ayca ; Eichenbaum, Kenneth D. ; Sadler Edepli, Kirsten. / A zebrafish retinal graded photochemical stress model. In: Journal of Pharmacological and Toxicological Methods. 2009 ; Vol. 59, No. 3. pp. 121-127.
    @article{267cedc504b34bad8c165ef170154bcc,
    title = "A zebrafish retinal graded photochemical stress model",
    abstract = "Introduction: In order to develop a model for investigating the genes that contribute to retinal degeneration, we examined the early graded photochemical stress response in the adult zebrafish (Danio rerio) retina and investigated the role of an NMDA inhibitor, thiokynurenate. Methods: Following intravitreal injection of rose bengal (6 or 12 mg/mL), light (37 × 103 or 83 × 103 lx) was directed onto the central retina with and without 400 nM thiokynurenate. Histologic and electron microscopic analysis was performed at 2 and 4 h and gene expression analysis was carried out at 2, 4 and 6 h. Results: Light and electron microscopy demonstrated a graded photochemical response in photoreceptor, nuclear, and ganglion cell layer thickness. Increased vacuolation of the inner plexiform layer was also observed. The inhibitor produced a distinct lesion pattern. Cellular stress genes were elevated in low and high lesions, while some homeobox gene expression was reduced with thiokynurenate. Discussion: The phenotypic and genetic changes observed from this model can serve as a basis for understanding the pathology of retinal oxidative and cellular stress. These changes may aid our understanding of aging and macular degeneration.",
    keywords = "Genes in retinal degeneration, NMDA inhibitor, Photochemical cellular stress, Zebrafish",
    author = "Eichenbaum, {Joseph W.} and Ayca Cinaroglu and Eichenbaum, {Kenneth D.} and {Sadler Edepli}, Kirsten",
    year = "2009",
    month = "5",
    day = "1",
    doi = "10.1016/j.vascn.2009.02.006",
    language = "English (US)",
    volume = "59",
    pages = "121--127",
    journal = "Journal of Pharmacological and Toxicological Methods",
    issn = "1056-8719",
    publisher = "Elsevier Inc.",
    number = "3",

    }

    TY - JOUR

    T1 - A zebrafish retinal graded photochemical stress model

    AU - Eichenbaum, Joseph W.

    AU - Cinaroglu, Ayca

    AU - Eichenbaum, Kenneth D.

    AU - Sadler Edepli, Kirsten

    PY - 2009/5/1

    Y1 - 2009/5/1

    N2 - Introduction: In order to develop a model for investigating the genes that contribute to retinal degeneration, we examined the early graded photochemical stress response in the adult zebrafish (Danio rerio) retina and investigated the role of an NMDA inhibitor, thiokynurenate. Methods: Following intravitreal injection of rose bengal (6 or 12 mg/mL), light (37 × 103 or 83 × 103 lx) was directed onto the central retina with and without 400 nM thiokynurenate. Histologic and electron microscopic analysis was performed at 2 and 4 h and gene expression analysis was carried out at 2, 4 and 6 h. Results: Light and electron microscopy demonstrated a graded photochemical response in photoreceptor, nuclear, and ganglion cell layer thickness. Increased vacuolation of the inner plexiform layer was also observed. The inhibitor produced a distinct lesion pattern. Cellular stress genes were elevated in low and high lesions, while some homeobox gene expression was reduced with thiokynurenate. Discussion: The phenotypic and genetic changes observed from this model can serve as a basis for understanding the pathology of retinal oxidative and cellular stress. These changes may aid our understanding of aging and macular degeneration.

    AB - Introduction: In order to develop a model for investigating the genes that contribute to retinal degeneration, we examined the early graded photochemical stress response in the adult zebrafish (Danio rerio) retina and investigated the role of an NMDA inhibitor, thiokynurenate. Methods: Following intravitreal injection of rose bengal (6 or 12 mg/mL), light (37 × 103 or 83 × 103 lx) was directed onto the central retina with and without 400 nM thiokynurenate. Histologic and electron microscopic analysis was performed at 2 and 4 h and gene expression analysis was carried out at 2, 4 and 6 h. Results: Light and electron microscopy demonstrated a graded photochemical response in photoreceptor, nuclear, and ganglion cell layer thickness. Increased vacuolation of the inner plexiform layer was also observed. The inhibitor produced a distinct lesion pattern. Cellular stress genes were elevated in low and high lesions, while some homeobox gene expression was reduced with thiokynurenate. Discussion: The phenotypic and genetic changes observed from this model can serve as a basis for understanding the pathology of retinal oxidative and cellular stress. These changes may aid our understanding of aging and macular degeneration.

    KW - Genes in retinal degeneration

    KW - NMDA inhibitor

    KW - Photochemical cellular stress

    KW - Zebrafish

    UR - http://www.scopus.com/inward/record.url?scp=67349269227&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=67349269227&partnerID=8YFLogxK

    U2 - 10.1016/j.vascn.2009.02.006

    DO - 10.1016/j.vascn.2009.02.006

    M3 - Article

    VL - 59

    SP - 121

    EP - 127

    JO - Journal of Pharmacological and Toxicological Methods

    JF - Journal of Pharmacological and Toxicological Methods

    SN - 1056-8719

    IS - 3

    ER -