A transcription factor hierarchy defines an environmental stress response network

Liang Song, Shao-Shan Huang, Aaron Wise, Rosa Castanoz, Joseph R. Nery, Huaming Chen, Marina Watanabe, Jerushah Thomas, Ziv Bar-Joseph, Joseph R. Ecker

Research output: Contribution to journalArticle

Abstract

Environmental stresses are universally encountered by microbes, plants, and animals.Yet systematic studies of stress-responsive transcription factor (TF) networks in multicellular organisms have been limited.The phytohormone abscisic acid (ABA) influences the expression of thousands of genes, allowing us to characterize complex stress-responsive regulatory networks. Using chromatin immunoprecipitation sequencing, we identified genome-wide targets of 21 ABA-related TFs to construct a comprehensive regulatory network in Arabidopsis thaliana. Determinants of dynamic TF binding and a hierarchy among TFs were defined, illuminating the relationship between differential gene expression patterns and ABA pathway feedback regulation. By extrapolating regulatory characteristics of observed canonical ABA pathway components, we identified a new family of transcriptional regulators modulating ABA and salt responsiveness and demonstrated their utility to modulate plant resilience to osmotic stress.

Original languageEnglish (US)
Article numberaag1550
JournalScience
Volume354
Issue number6312
DOIs
StatePublished - Nov 4 2016

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Abscisic Acid
Transcription Factors
Gene Expression
Plant Growth Regulators
Chromatin Immunoprecipitation
Osmotic Pressure
Arabidopsis
Salts
Genome

ASJC Scopus subject areas

  • General

Cite this

Song, L., Huang, S-S., Wise, A., Castanoz, R., Nery, J. R., Chen, H., ... Ecker, J. R. (2016). A transcription factor hierarchy defines an environmental stress response network. Science, 354(6312), [aag1550]. https://doi.org/10.1126/science.aag1550

A transcription factor hierarchy defines an environmental stress response network. / Song, Liang; Huang, Shao-Shan; Wise, Aaron; Castanoz, Rosa; Nery, Joseph R.; Chen, Huaming; Watanabe, Marina; Thomas, Jerushah; Bar-Joseph, Ziv; Ecker, Joseph R.

In: Science, Vol. 354, No. 6312, aag1550, 04.11.2016.

Research output: Contribution to journalArticle

Song, L, Huang, S-S, Wise, A, Castanoz, R, Nery, JR, Chen, H, Watanabe, M, Thomas, J, Bar-Joseph, Z & Ecker, JR 2016, 'A transcription factor hierarchy defines an environmental stress response network', Science, vol. 354, no. 6312, aag1550. https://doi.org/10.1126/science.aag1550
Song L, Huang S-S, Wise A, Castanoz R, Nery JR, Chen H et al. A transcription factor hierarchy defines an environmental stress response network. Science. 2016 Nov 4;354(6312). aag1550. https://doi.org/10.1126/science.aag1550
Song, Liang ; Huang, Shao-Shan ; Wise, Aaron ; Castanoz, Rosa ; Nery, Joseph R. ; Chen, Huaming ; Watanabe, Marina ; Thomas, Jerushah ; Bar-Joseph, Ziv ; Ecker, Joseph R. / A transcription factor hierarchy defines an environmental stress response network. In: Science. 2016 ; Vol. 354, No. 6312.
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