A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids

Changchun Liu, Xianbo Qiu, Serge Ongagna, Dafeng Chen, Zongyuan Chen, William R. Abrams, Daniel Malamud, Paul L A M Corstjens, Haim H. Bau

Research output: Contribution to journalArticle

Abstract

An inexpensive, hand-held, point-of-care, disposable, self-contained immunoassay cassette comprised of air pouches for pumping, a metering chamber, reagents storage chambers, a mixer, and a lateral flow strip was designed, constructed, and tested. The assay was carried out in a consecutive flow format. The detection was facilitated with up-converting phosphor (UCP) reporter particles. The automated, timely pumping of the various reagents was driven by a spring-loaded timer. The utility of the cassette was demonstrated by detecting antibodies to HIV in saliva samples and further evaluated with a non-contagious, haptenized DNA assay. The cassette has several advantages over dip sticks such as sample preprocessing, integrated storage of reagents, and automated operation that reduces operator errors and training. The cassette and actuator described herein can readily be extended to detect biomarkers of other diseases in body fluids and other fluids at the point of care. The system is particularly suitable for resource-poor countries, where funds and trained personnel are in short supply.

Original languageEnglish (US)
Pages (from-to)768-776
Number of pages9
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume9
Issue number6
DOIs
StatePublished - 2009

Fingerprint

Point-of-Care Systems
Immunoassay
Assays
Fluids
Body fluids
Biomarkers
Antibodies
Phosphors
HIV Antibodies
DNA
Actuators
Body Fluids
Personnel
Financial Management
Saliva
Hand
Air

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids. / Liu, Changchun; Qiu, Xianbo; Ongagna, Serge; Chen, Dafeng; Chen, Zongyuan; Abrams, William R.; Malamud, Daniel; Corstjens, Paul L A M; Bau, Haim H.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 9, No. 6, 2009, p. 768-776.

Research output: Contribution to journalArticle

Liu, C, Qiu, X, Ongagna, S, Chen, D, Chen, Z, Abrams, WR, Malamud, D, Corstjens, PLAM & Bau, HH 2009, 'A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids', Lab on a Chip - Miniaturisation for Chemistry and Biology, vol. 9, no. 6, pp. 768-776. https://doi.org/10.1039/b814322f
Liu, Changchun ; Qiu, Xianbo ; Ongagna, Serge ; Chen, Dafeng ; Chen, Zongyuan ; Abrams, William R. ; Malamud, Daniel ; Corstjens, Paul L A M ; Bau, Haim H. / A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2009 ; Vol. 9, No. 6. pp. 768-776.
@article{fe4d2ca65a8b47ba8408ba72ee7fe6e3,
title = "A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids",
abstract = "An inexpensive, hand-held, point-of-care, disposable, self-contained immunoassay cassette comprised of air pouches for pumping, a metering chamber, reagents storage chambers, a mixer, and a lateral flow strip was designed, constructed, and tested. The assay was carried out in a consecutive flow format. The detection was facilitated with up-converting phosphor (UCP) reporter particles. The automated, timely pumping of the various reagents was driven by a spring-loaded timer. The utility of the cassette was demonstrated by detecting antibodies to HIV in saliva samples and further evaluated with a non-contagious, haptenized DNA assay. The cassette has several advantages over dip sticks such as sample preprocessing, integrated storage of reagents, and automated operation that reduces operator errors and training. The cassette and actuator described herein can readily be extended to detect biomarkers of other diseases in body fluids and other fluids at the point of care. The system is particularly suitable for resource-poor countries, where funds and trained personnel are in short supply.",
author = "Changchun Liu and Xianbo Qiu and Serge Ongagna and Dafeng Chen and Zongyuan Chen and Abrams, {William R.} and Daniel Malamud and Corstjens, {Paul L A M} and Bau, {Haim H.}",
year = "2009",
doi = "10.1039/b814322f",
language = "English (US)",
volume = "9",
pages = "768--776",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "6",

}

TY - JOUR

T1 - A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids

AU - Liu, Changchun

AU - Qiu, Xianbo

AU - Ongagna, Serge

AU - Chen, Dafeng

AU - Chen, Zongyuan

AU - Abrams, William R.

AU - Malamud, Daniel

AU - Corstjens, Paul L A M

AU - Bau, Haim H.

PY - 2009

Y1 - 2009

N2 - An inexpensive, hand-held, point-of-care, disposable, self-contained immunoassay cassette comprised of air pouches for pumping, a metering chamber, reagents storage chambers, a mixer, and a lateral flow strip was designed, constructed, and tested. The assay was carried out in a consecutive flow format. The detection was facilitated with up-converting phosphor (UCP) reporter particles. The automated, timely pumping of the various reagents was driven by a spring-loaded timer. The utility of the cassette was demonstrated by detecting antibodies to HIV in saliva samples and further evaluated with a non-contagious, haptenized DNA assay. The cassette has several advantages over dip sticks such as sample preprocessing, integrated storage of reagents, and automated operation that reduces operator errors and training. The cassette and actuator described herein can readily be extended to detect biomarkers of other diseases in body fluids and other fluids at the point of care. The system is particularly suitable for resource-poor countries, where funds and trained personnel are in short supply.

AB - An inexpensive, hand-held, point-of-care, disposable, self-contained immunoassay cassette comprised of air pouches for pumping, a metering chamber, reagents storage chambers, a mixer, and a lateral flow strip was designed, constructed, and tested. The assay was carried out in a consecutive flow format. The detection was facilitated with up-converting phosphor (UCP) reporter particles. The automated, timely pumping of the various reagents was driven by a spring-loaded timer. The utility of the cassette was demonstrated by detecting antibodies to HIV in saliva samples and further evaluated with a non-contagious, haptenized DNA assay. The cassette has several advantages over dip sticks such as sample preprocessing, integrated storage of reagents, and automated operation that reduces operator errors and training. The cassette and actuator described herein can readily be extended to detect biomarkers of other diseases in body fluids and other fluids at the point of care. The system is particularly suitable for resource-poor countries, where funds and trained personnel are in short supply.

UR - http://www.scopus.com/inward/record.url?scp=61849104523&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61849104523&partnerID=8YFLogxK

U2 - 10.1039/b814322f

DO - 10.1039/b814322f

M3 - Article

C2 - 19255658

AN - SCOPUS:61849104523

VL - 9

SP - 768

EP - 776

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0197

IS - 6

ER -