A three-dimensional homology model of the O-acetylserine sulfhydrylase-B from Salmonella typhimurium

Wael Rabeh, Timothy Mather, Paul F. Cook

    Research output: Contribution to journalArticle

    Abstract

    O-acetylserine sulfhydrylase (OASS) catalyzes the last step in the cysteine biosynthetic pathway in enteric bacteria and plants. The overall pathway involves the substitution of the β-acetoxy group of O-acetyl-L-serine with inorganic bisulfide. Two isozymes are present in S. typhimurium, the A- and B-isozymes, expressed under aerobic and anaerobic conditions, respectively. No crystal structure is presently available for the B-isozyme. Kinetic data indicate the catalytic mechanism of OASS-B is ping-pong, as found for the A-isozyme, but kinetic parameters and substrate specificity differ. In order to estimate whether structural differences may be responsible for the kinetic differences, a homology model was built using the structure of OASS-A as the template for the OASS-B model. The β-subunit of tryptophan synthase and cystathionine β-synthase were used for comparison. Differences between the OASS-A structure and the homology model for OASS-B are discussed.

    Original languageEnglish (US)
    Pages (from-to)7-13
    Number of pages7
    JournalProtein and Peptide Letters
    Volume13
    Issue number1
    DOIs
    StatePublished - Jan 1 2006

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    Cysteine Synthase
    Salmonella
    Salmonella typhimurium
    Isoenzymes
    Tryptophan Synthase
    Cystathionine
    Kinetics
    Biosynthetic Pathways
    Enterobacteriaceae
    Substrate Specificity
    Kinetic parameters
    Serine
    Cysteine
    Bacteria
    Substitution reactions
    Crystal structure
    Substrates

    ASJC Scopus subject areas

    • Structural Biology
    • Biochemistry

    Cite this

    A three-dimensional homology model of the O-acetylserine sulfhydrylase-B from Salmonella typhimurium. / Rabeh, Wael; Mather, Timothy; Cook, Paul F.

    In: Protein and Peptide Letters, Vol. 13, No. 1, 01.01.2006, p. 7-13.

    Research output: Contribution to journalArticle

    Rabeh, Wael ; Mather, Timothy ; Cook, Paul F. / A three-dimensional homology model of the O-acetylserine sulfhydrylase-B from Salmonella typhimurium. In: Protein and Peptide Letters. 2006 ; Vol. 13, No. 1. pp. 7-13.
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