A system of repressor gradients spatially organizes the boundaries of bicoid-dependent target genes

Hongtao Chen, Zhe Xu, Constance Mei, Danyang Yu, Stephen Small

Research output: Contribution to journalArticle

Abstract

The homeodomain (HD) protein Bicoid (Bcd) is thought to function as a gradient morphogen that positions boundaries of target genes via threshold-dependent activation mechanisms. Here, we analyze 66 Bcd-dependent regulatory elements and show that their boundaries are positioned primarily by repressive gradients that antagonize Bcd-mediated activation. A major repressor is the pair-rule protein Runt (Run), which is expressed in an opposing gradient and is necessary and sufficient for limiting Bcd-dependent activation. Evidence is presented that Run functions with the maternal repressor Capicua and the gap protein Kruppel as the principal components of a repression system that correctly orders boundaries throughout the anterior half of the embryo. These results put conceptual limits on the Bcd morphogen hypothesis and demonstrate how the Bcd gradient functions within the gene network that patterns the embryo.

Original languageEnglish (US)
Pages (from-to)618-629
Number of pages12
JournalCell
Volume149
Issue number3
DOIs
StatePublished - Apr 27 2012

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Embryonic Structures
Genes
Chemical activation
Homeodomain Proteins
Gene Regulatory Networks
Proteins
Mothers

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A system of repressor gradients spatially organizes the boundaries of bicoid-dependent target genes. / Chen, Hongtao; Xu, Zhe; Mei, Constance; Yu, Danyang; Small, Stephen.

In: Cell, Vol. 149, No. 3, 27.04.2012, p. 618-629.

Research output: Contribution to journalArticle

Chen, Hongtao ; Xu, Zhe ; Mei, Constance ; Yu, Danyang ; Small, Stephen. / A system of repressor gradients spatially organizes the boundaries of bicoid-dependent target genes. In: Cell. 2012 ; Vol. 149, No. 3. pp. 618-629.
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